植物乳杆菌三种质粒的功能分析
Functional analysis of three plasmids from Lactobacillus plantarum.
作者信息
van Kranenburg Richard, Golic Natasa, Bongers Roger, Leer Rob J, de Vos Willem M, Siezen Roland J, Kleerebezem Michiel
机构信息
Wageningen Centre for Food Sciences, Wageningen, The Netherlands.
出版信息
Appl Environ Microbiol. 2005 Mar;71(3):1223-30. doi: 10.1128/AEM.71.3.1223-1230.2005.
Abstract
Lactobacillus plantarum WCFS1 harbors three plasmids, pWCFS101, pWCFS102, and pWCFS103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. The two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. The host range of the pWCFS101 replicon includes Lactobacillus species and Lactococcus lactis, while that of the pWCFS102 replicon also includes Carnobacterium maltaromaticum and Bacillus subtilis. The larger plasmid is predicted to replicate via the theta-type mechanism. The host range of its replicon seems restricted to L. plantarum. Cloning vectors were constructed based on the replicons of all three plasmids. Plasmid pWCFS103 was demonstrated to be a conjugative plasmid, as it could be transferred to L. plantarum NC8. It confers arsenate and arsenite resistance, which can be used as selective markers.
摘要
植物乳杆菌WCFS1含有三个质粒，分别为pWCFS101、pWCFS102和pWCFS103，大小分别为1917 bp、2365 bp和36069 bp。两个较小的质粒功能未知，含有可能通过滚环复制机制发挥作用的复制基因。pWCFS101复制子的宿主范围包括植物乳杆菌属和乳酸乳球菌，而pWCFS102复制子的宿主范围还包括麦芽香肉杆菌和枯草芽孢杆菌。较大的质粒预计通过θ型机制复制。其复制子的宿主范围似乎仅限于植物乳杆菌。基于所有三个质粒的复制子构建了克隆载体。质粒pWCFS103被证明是一种接合性质粒，因为它可以转移到植物乳杆菌NC8中。它赋予对砷酸盐和亚砷酸盐的抗性，可作为选择标记。
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