Rodríguez Alba I, Willing Alison E, Cameron Don F, Saporta Samuel, Sandberg Paul R
Department of Neurosurgery and The Neurosciences Program, Center for Aging and Brain Repair, College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Neurotox Res. 2002 Mar;4(2):103-9. doi: 10.1080/10298420290015935.
Sertoli cells, a testes-derived cell with immunosuppressive and trophic properties, may serve as an alternative cell source for transplantation in a number of neurodegenerative diseases. However, before Sertoli cells can be considered for clinical use, safety studies must be conducted to ensure that the cells themselves produce no adverse effects when transplanted into the central nervous system. The present study assessed the behavioral effects of transplanting porcine Sertoli cells into the striatum of normal rats and provided a histological examination of the graft site and host striatum. Activity monitors revealed significant increases in nocturnal locomotor activity over time following both sham and Sertoli transplants. Ambulation and rearing, but not stereotypic measures, were increased compared to pre-transplant levels. Sertoli animals exhibited less behavioral alteration than sham controls. Histological examination of the striatum demonstrated surviving Sertoli cell transplants in an intact striatum. These results indicated that Sertoli cell xenografts might be a safe alternative cell source for neurotransplantation procedures requiring immune or trophic support.
支持细胞是一种源自睾丸的具有免疫抑制和营养特性的细胞，在多种神经退行性疾病中，它可能作为移植的替代细胞来源。然而，在考虑将支持细胞用于临床之前，必须进行安全性研究，以确保细胞移植到中枢神经系统时自身不会产生不良影响。本研究评估了将猪支持细胞移植到正常大鼠纹状体中的行为影响，并对移植部位和宿主纹状体进行了组织学检查。活动监测显示，假手术和支持细胞移植后，夜间运动活动随时间显著增加。与移植前水平相比，行走和竖毛行为增加，但刻板行为未增加。接受支持细胞移植的动物比假手术对照组表现出更少的行为改变。纹状体的组织学检查表明，完整的纹状体中有存活的支持细胞移植。这些结果表明，支持细胞异种移植可能是需要免疫或营养支持的神经移植手术的一种安全的替代细胞来源。
