Lyng R D
Department of Biology, Indiana University-Purdue University, Fort Wayne 46805.
Toxicol Lett. 1992 Dec;63(3):289-98. doi: 10.1016/0378-4274(92)90091-w.
Culture systems offer advantages for studying the actions of chemicals on mechanisms of development. Growth, [3H]thymidine incorporation, and 14C-amino acid incorporation were measured to determine culture conditions and the response of glands to three chemicals. Eagle's MEM supported the best growth and the best time for double isotope presence was the second 24 h of culture. Cyclamate had no effect on growth or isotope incorporation but cycloheximide disturbed 14C more than 3H. 5-Azacytidine was toxic but the incorporation of both isotopes exhibited the same pattern.
培养系统为研究化学物质对发育机制的作用提供了优势。通过测量生长、[3H]胸腺嘧啶核苷掺入量和14C-氨基酸掺入量来确定培养条件以及腺体对三种化学物质的反应。伊格尔氏最低限度基本培养基支持最佳生长，双同位素存在的最佳时间是培养的第二个24小时。甜蜜素对生长或同位素掺入没有影响，但环己酰亚胺对14C的干扰比对3H的干扰更大。5-氮杂胞苷具有毒性，但两种同位素的掺入呈现相同模式。
