Kjelgaard-Hansen Mads, Jensen Asger Lundorff, Kristensen Annemarie T
Central Laboratory and the Small Animal Hospital, Department of Clinical Studies, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg, Denmark.
Vet Clin Pathol. 2003;32(2):81-7. doi: 10.1111/j.1939-165x.2003.tb00319.x.
Serum C-reactive protein (CRP) is an acute phase marker in dogs that is useful for the diagnosis and monitoring of inflammatory disease. Rapid, reliable, and automated assays are preferable for routine evaluation of canine serum CRP concentration.
The aim of this study was to evaluate whether canine serum CRP concentration could be measured reliably using an automated turbidometric immunoassay (TIA) designed for use with human serum.
A commercially available TIA for human serum CRP (Bayer, Newbury, UK) was used to measure canine serum CRP concentration. Cross-reactivity of antigen was evaluated by the Ouchterlony procedure. Intra- and interassay imprecision was investigated by multiple measurements on canine serum samples and serum pools, respectively. Assay inaccuracy was investigated by linearity under dilution and comparison of methodologies (canine CRP ELISA, Tridelta Development Ltd, Kildare, UK). Then the assay was applied to serum samples from 14 clinically healthy dogs, 11 dogs with neoplasia, 13 with infections, 8 with endocrine or metabolic diseases, and 10 with miscellaneous diseases.
Cross-reactivity between canine serum CRP and the anti-human CRP antibody was found. Intra- and interassay imprecision ranged from 5.2% to 10.8% and 3.0% to 10.2%, respectively. Serum CRP concentration was measured in a linear and proportional manner. There was no significant disagreement and there was linear correlation of the results in the comparison of methodologies, except for a slight proportional discrepancy at low CRP concentrations (<10 microg/mL). Dogs with infections had a significantly higher concentration of serum CRP than did all other dogs, and dogs with neoplasia had a significantly higher concentration of serum CRP than did clinically healthy dogs.
Canine serum CRP concentration can be measured reliably using the commercially available TIA designed for human CRP.
血清C反应蛋白（CRP）是犬类的一种急性期标志物，可用于炎症性疾病的诊断和监测。快速、可靠且自动化的检测方法对于犬血清CRP浓度的常规评估更为可取。
本研究的目的是评估使用专为检测人血清而设计的自动比浊免疫分析法（TIA）能否可靠地检测犬血清CRP浓度。
使用一种市售的人血清CRP TIA（英国纽伯里的拜耳公司）来检测犬血清CRP浓度。通过双向免疫扩散法评估抗原的交叉反应性。分别对犬血清样本和血清混合液进行多次测量，以研究批内和批间不精密度。通过稀释线性度和方法比较（犬CRP酶联免疫吸附测定法，英国基尔代尔的Tridelta Development Ltd公司）来研究检测不准确度。然后将该检测方法应用于14只临床健康犬、11只患有肿瘤的犬、13只患有感染的犬、8只患有内分泌或代谢疾病的犬以及10只患有其他杂症的犬的血清样本。
发现犬血清CRP与人抗CRP抗体之间存在交叉反应。批内和批间不精密度分别为5.2%至10.8%和3.0%至10.2%。血清CRP浓度呈线性和比例性测量。在方法比较中，除了低CRP浓度（<10微克/毫升）时有轻微的比例差异外，结果没有显著差异且呈线性相关。患有感染的犬血清CRP浓度显著高于所有其他犬，患有肿瘤的犬血清CRP浓度显著高于临床健康犬。
使用市售的人CRP TIA能够可靠地检测犬血清CRP浓度。
