Lundgren C, Frankendal B, Silfverswärd C, Nilsson B, Tryggvason K, Auer G, Nordström B
Department of Gynaecologic Oncology, Radiumhemmet, Karolinska Hospital and Institute, S 171-76 Stockholm, Sweden.
Med Oncol. 2003;20(2):147-56. doi: 10.1385/MO:20:2:147.
Expression of the laminin-5 gamma2-chain in carcinoma cells has been implicated in tumor invasion. The aim was to investigate the expression and prognostic significance of the ln-5 gamma2-chain compared with clinicopathological factors and tumor cell DNA ploidy in endometrial carcinoma. Histological specimens from 80 endometrial carcinomas were examined with respect to immunohistochemical ln-5 gamma2-chain expression and correlated to the clinicopathological characteristics, DNA ploidy, and survival. Sixty-eight of 80 investigated cases were judged to be positive for the ln-5 gamma2-chain. Ln-5 gamma2-chain did not show any correlation to stage, histopathological subtype, grade, and DNA ploidy. In univariate analyses, advanced stage (p < 0.001), nonendometrioid carcinoma (p = 0.030), low grade (p < 0.001), aneuploid tumors (p < 0.001), and ln-5 gamma2-chain expression (p = 0.017) were highly associated with poor survival. Aneuploid tumors in combination with strong ln-5 gamma2-chain expression were significant predictors (p < 0.001) of poor prognosis. In multivariate analyses including stage, histopathological subgroup, grade, DNA ploidy, and ln-5 gamma2-chain expression, all lost their significant prognostic information except for stage (p < 0.001) and grade (p < 0.05). Ln-5 gamma2-chain expression and DNA ploidy both as a single parameter and in combination were demonstrated to be signifi- cant prognostic factors in univariate analysis. However, stage and grade provided more useful clinical information beyond histopathological subgroup, DNA ploidy, and ln-5 gamma2-chain expression. The results also indicate that ln-5 gamma2-chain expression is upregulated during the progression of endometrial carcinoma.
层粘连蛋白-5γ2链在癌细胞中的表达与肿瘤侵袭有关。目的是研究层粘连蛋白-5γ2链与子宫内膜癌临床病理因素及肿瘤细胞DNA倍体相比的表达情况及预后意义。对80例子宫内膜癌的组织学标本进行免疫组织化学检测，观察层粘连蛋白-5γ2链的表达情况，并与临床病理特征、DNA倍体及生存率进行相关性分析。80例研究病例中有68例被判定为层粘连蛋白-5γ2链阳性。层粘连蛋白-5γ2链与分期、组织病理学亚型、分级及DNA倍体均无相关性。单因素分析显示，晚期（p<0.001）、非子宫内膜样癌（p=0.030）、低分级（p<0.001）、非整倍体肿瘤（p<0.001）及层粘连蛋白-5γ2链表达（p=0.017）均与生存率低密切相关。非整倍体肿瘤与层粘连蛋白-5γ2链强表达联合是预后不良的重要预测指标（p<0.001）。多因素分析包括分期、组织病理学亚组、分级、DNA倍体及层粘连蛋白-5γ2链表达，除分期（p<0.001）和分级（p<0.05）外，其他因素均失去其显著的预后信息。在单因素分析中，层粘连蛋白-5γ2链表达及DNA倍体单独及联合均被证明是重要的预后因素。然而，分期和分级比组织病理学亚组、DNA倍体及层粘连蛋白-5γ2链表达提供了更有用的临床信息。结果还表明，在子宫内膜癌进展过程中层粘连蛋白-5γ2链表达上调。
