Maillard J, Favreau C
Br J Exp Pathol. 1975 Apr;56(2):139-47.
Current ways of fractionating immune sera give rise in the guinea-pig species to an active plasmin that contaminates antibody preparations. The enzyme appears as a free molecule separable from the immunoglobulin. In contrast, when a purified antibody has been obtained through the immunoabsorption process, plasmin activated during the contact between antibody and polymer binds firmly to the immunoglobulin. This phenomenon occurs separately and indistinctly with IgG1 and IgG2. Moreover, the alternate pathway of complement activation could be a tributary to the plasmin system.
目前对免疫血清进行分级分离的方法，在豚鼠物种中会产生一种污染抗体制剂的活性纤溶酶。该酶以可与免疫球蛋白分离的游离分子形式出现。相比之下，当通过免疫吸附过程获得纯化抗体时，抗体与聚合物接触期间激活的纤溶酶会牢固地结合到免疫球蛋白上。这种现象在IgG1和IgG2中分别且不明显地发生。此外，补体激活的替代途径可能是纤溶酶系统的一个支流。
