Gopen Quinton, Lopez Ivan, Ishiyama Gail, Baloh Robert W, Ishiyama Akira
Division of Head & Neck Surgery, Medical Center, University of California-Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA.
Laryngoscope. 2003 Jul;113(7):1132-8. doi: 10.1097/00005537-200307000-00007.
The objective of the study was to obtain unbiased estimates of the total number of type I and type II hair cells in human utricular macula from individuals with documented normal vestibular function.
Application of unbiased stereology using microdissected human temporal bone specimens was conducted in an observational study.
Postmortem temporal bones were obtained from 10 normal patients (age range, 42-96 y; mean age, 82 y). The utricular maculae were microdissected, embedded in plastic, and cut into serial 2-microm sections. Unbiased estimates of the total number of type I and type II hair cells were obtained using the physical fractionator technique of stereology.
The average total number of hair cells was 27,508 (CV = 11%) consisting of 17,326 (coefficient of variation [CV] = 11%) type I hair cells and 10,182 (CV = 13%) type II hair cells. The ratio of type I to type II hair cells was 1.70:1. In the age range of the study, there was no statistically significant correlation between hair cell counts and age.
Morphometric studies of the human utricular sensory epithelium can be accomplished using unbiased stereology on microdissected specimens. There was no effect of age on total hair cell counts or on the ratio of type I to type II hair cells in the age range of the study. Further studies on younger subjects are needed to establish the effect of age. The results from the present study are closely aligned with prior studies that estimated total hair cell counts using surface mount preparations. The current data represent the first total type I and type II hair cell counts in human utricular neuroepithelium.
本研究的目的是对前庭功能记录正常的个体的人椭圆囊斑中I型和II型毛细胞的总数进行无偏估计。
在一项观察性研究中，对显微解剖的人颞骨标本应用无偏体视学方法。
从10名正常患者（年龄范围42 - 96岁；平均年龄82岁）获取死后颞骨。将椭圆囊斑显微解剖，包埋于塑料中，并切成2微米的连续切片。使用体视学的物理分割器技术获得I型和II型毛细胞总数的无偏估计。
毛细胞的平均总数为27,508个（变异系数[CV]=11%），其中I型毛细胞17,326个（变异系数[CV]=11%），II型毛细胞10,182个（CV = 13%）。I型与II型毛细胞的比例为1.70:1。在本研究的年龄范围内，毛细胞计数与年龄之间无统计学显著相关性。
对人椭圆囊感觉上皮的形态计量学研究可以通过对显微解剖标本应用无偏体视学来完成。在本研究的年龄范围内，年龄对毛细胞总数或I型与II型毛细胞的比例没有影响。需要对更年轻的受试者进行进一步研究以确定年龄的影响。本研究结果与先前使用表面贴装制剂估计毛细胞总数的研究密切一致。目前的数据代表了人椭圆囊神经上皮中I型和II型毛细胞总数的首次统计。
