Yonezawa M, Otsuka T, Matsui N, Tsuji H, Kato K H, Moriyama A, Kato T
Department of Orthopedic Surgery, Nagoya City University Medical School, Japan.
Int J Cancer. 1996 May 3;66(3):347-51. doi: 10.1002/(SICI)1097-0215(19960503)66:3<347::AID-IJC14>3.0.CO;2-8.
The effect of mild hyperthermia on a cultured rat malignant fibrous histiocytoma (MFH) cell line, MFH-2NR, was investigated. MFH cells in log-phase (growing phase) were heated at 41 degrees-44 degrees C for 1 hr. Hyperthermic treatment at 41 degrees C did not substantially affect cell proliferation and treatment at 44 degrees C caused necrosis. After hypothermic treatment at 42 degrees or 43 degrees C, proliferation of MFH cells was arrested and morphological changes characteristic of apoptosis, cell shrinkage accompanying apoptotic bodies and chromatin condensation, became apparent. Hyperthermia-induced apoptosis was further confirmed by terminal deoxynucleotidyl transferase staining and a ladder pattern on agarose gel electrophoresis. Flow cytometric analysis indicated that the population in the G1 phase of the cell cycle significantly decreased with a concomitant increase in apoptotic cells, indicating that apoptosis might occur mainly in the G1 phase population.
研究了轻度热疗对培养的大鼠恶性纤维组织细胞瘤（MFH）细胞系MFH-2NR的影响。对数期（生长阶段）的MFH细胞在41℃-44℃加热1小时。41℃的热疗对细胞增殖没有实质性影响，而44℃的热疗导致细胞坏死。在42℃或43℃进行低温治疗后，MFH细胞的增殖被抑制，凋亡的形态学变化变得明显，包括细胞收缩伴随凋亡小体和染色质凝聚。末端脱氧核苷酸转移酶染色和琼脂糖凝胶电泳上的梯状条带进一步证实了热疗诱导的凋亡。流式细胞术分析表明，细胞周期G1期的细胞群体显著减少，同时凋亡细胞增加，表明凋亡可能主要发生在G1期群体中。
