Fainaru Ofer, Lichtenberg Dov, Pinchuk Ilya, Almog Benny, Gamzu Ronni, Kupferminc Michael
Department of Obstetrics and Gynecology, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Israel.
Acta Obstet Gynecol Scand. 2003 Aug;82(8):711-5. doi: 10.1034/j.1600-0412.2003.00182.x.
Several reports suggest preeclampsia to be associated with oxidative stress. In view of potential experimental artifacts in these studies, we tested the effect of preeclampsia on the oxidizibility of maternal serum lipids, using an optimized ex vivo method.
This prospective study included 28 pregnant women with preeclampsia and 28 women matched for maternal and gestational age with normal pregnancies. Venous blood was drawn from the consenting women. Serum levels of lipid peroxidation products and the kinetics of copper-induced oxidation ex vivo were monitored spectroscopically by continuous recording of absorbance at 245 nm.
The initial optical density (OD) at 245 nm, attributed to preformed products of lipid peroxidation, was higher in the women with preeclampsia than in the controls (1.26 +/- 0.02 vs. 1.17 +/- 0.02 OD units; p = 0.01). The lag phase preceding oxidation, reflecting resistance of serum lipids to oxidation, was significantly shorter in the preeclampsia group than in the controls (47.4 +/- 2.3 vs. 57.6 +/- 4.0 min; p = 0.027).
High levels of serum hydroperoxides and increased susceptibility of serum lipids to copper-induced peroxidation ex vivo indicate preeclampsia to be associated with high oxidative stress. The role of this high oxidizibility in the pathogenesis of preeclampsia has yet to be evaluated.
多项报告表明子痫前期与氧化应激有关。鉴于这些研究中可能存在的实验假象，我们采用优化的体外方法测试了子痫前期对母体血清脂质氧化能力的影响。
这项前瞻性研究纳入了28例子痫前期孕妇和28例年龄及孕周匹配的正常妊娠妇女。从签署知情同意书的妇女中采集静脉血。通过连续记录245nm处的吸光度，用光谱法监测血清脂质过氧化产物水平及体外铜诱导氧化的动力学过程。
子痫前期组妇女中归因于脂质过氧化预制产物的245nm处初始光密度（OD）高于对照组（分别为1.26±0.02和1.17±0.02OD单位；p = 0.01）。反映血清脂质抗氧化能力的氧化前延迟期，子痫前期组明显短于对照组（分别为47.4±2.3和57.6±4.0分钟；p = 0.027）。
血清氢过氧化物水平升高以及体外血清脂质对铜诱导过氧化的敏感性增加表明子痫前期与高氧化应激有关。这种高氧化能力在子痫前期发病机制中的作用尚待评估。
