Fainaru Ofer, Almog Ronit, Pinchuk Ilya, Lichtenberg Dov, Lessing Joseph B, Kupferminc Michael J
Department of Obstetrics and Gynecology, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv University, Tel Aviv, Israel.
Department of Obstetrics and Gynecology, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv University, Tel Aviv, Israel.
Eur J Obstet Gynecol Reprod Biol. 2007 Mar;131(1):28-31. doi: 10.1016/j.ejogrb.2006.04.013. Epub 2006 May 23.
In our previous studies we have shown that the process of term labor is associated with oxidative stress, as indicated by increased susceptibility of maternal serum lipids to copper induced peroxidation. In order to continue evaluating the role of oxidative stress in the labor process, we next tested whether term premature rupture of the membranes (PROM) is also associated with increased susceptibility of maternal serum lipids to copper induced peroxidation.
A controlled prospective study.
Tertiary care centre.
31 healthy women with term PROM and 19 healthy pregnant women with intact membranes. The women were matched for maternal and gestational age.
Venous blood was drawn from the women (up to 6h after rupture of the membranes and prior to labor in the PROM group), and the kinetics of copper-induced oxidation of serum lipids ex vivo were monitored spectroscopically at 37 degrees C by continuous recording of absorbance at 245 nm.
The lag phase, reflecting resistance of serum lipids to oxidation, was similar in the PROM group when compared to the control group (43.7+/-3.2 versus 41.9+/-1.6 min, P=0.61). However, the maximal rate of oxidation (V(max)) and the maximal accumulation of absorbing products (OD(max)) were shorter in the PROM group when compared to the control group (5.14+/-0.26 versus 6.29+/-0.4010(-3) OD(245) nm/min, P=0.016; 0.61+/-0.03 versus 0.71+/-0.04 OD(245) nm, P=0.07).
As opposed to term labor, term PROM is not associated with increased maternal systemic oxidative stress when compared to normal pregnant women. The role for oxidative stress in preterm PROM warrants further studies.
在我们之前的研究中，我们已经表明足月分娩过程与氧化应激相关，这表现为母体血清脂质对铜诱导的过氧化作用的敏感性增加。为了继续评估氧化应激在分娩过程中的作用，我们接下来测试足月胎膜早破（PROM）是否也与母体血清脂质对铜诱导的过氧化作用的敏感性增加相关。
一项对照前瞻性研究。
三级护理中心。
31名足月胎膜早破的健康女性和19名胎膜完整的健康孕妇。这些女性在产妇年龄和孕周方面相匹配。
从这些女性中采集静脉血（胎膜早破组在胎膜破裂后6小时内且临产前，胎膜完整组在临产前），并通过在37℃下连续记录245nm处的吸光度，以光谱法监测体外铜诱导的血清脂质氧化动力学。
反映血清脂质抗氧化能力的延迟期，胎膜早破组与对照组相似（43.7±3.2分钟对41.9±1.6分钟，P = 0.61）。然而，与对照组相比，胎膜早破组的最大氧化速率（V(max)）和吸收产物的最大积累量（OD(max)）较短（5.14±0.26对6.29±0.40×10⁻³ OD(245)nm/分钟，P = 0.016；0.61±0.03对0.71±0.04 OD(245)nm，P = 0.07）。
与足月分娩不同，与正常孕妇相比，足月胎膜早破与母体全身氧化应激增加无关。氧化应激在早产胎膜早破中的作用值得进一步研究。
