Zhang Lian-Dong, Li He-Cheng, Chong Tie, Gao Ming, Yin Jian, Fu De-Lai, Deng Qian, Wang Zi-Ming
Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China.
Department of Nephrology, Xi'an No. 4 Hospital, Xi'an, Shaanxi 710004, China.
Biomed Res Int. 2014;2014:598630. doi: 10.1155/2014/598630. Epub 2014 Oct 29.
Di-(2-ethylhexyl) phthalate (DEHP) is the most widely used plastizer in the world and can suppress testosterone production via activation of oxidative stress. Genistein (GEN) is one of the isoflavones ingredients exhibiting weak estrogenic and potentially antioxidative effects. However, study on reproductive effects following prepubertal multiple endocrine disrupters exposure has been lacking. In this study, DEHP and GEN were administrated to prepubertal male Sprague-Dawley rats by gavage from postnatal day 22 (PND22) to PND35 with vehicle control, GEN at 50 mg/kg body weight (bw)/day (G), DEHP at 50, 150, 450 mg/kg bw/day (D50, D150, D450) and their mixture (G + D50, G + D150, G + D450). On PND90, general morphometry (body weight, AGD, organ weight, and organ coefficient), testicular redox state, and testicular histology were studied. Our results indicated that DEHP could significantly decrease sex organs weight, organ coefficient, and testicular antioxidative ability, which largely depended on the dose of DEHP. However, coadministration of GEN could partially alleviate DEHP-induced reproductive injuries via enhancement of testicular antioxidative enzymes activities, which indicates that GEN has protective effects on DEHP-induced male reproductive system damage after prepubertal exposure and GEN may have promising future in its curative antioxidative role for reproductive disorders caused by other environmental endocrine disruptors.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是全球使用最广泛的增塑剂，可通过激活氧化应激来抑制睾酮生成。染料木黄酮（GEN）是具有弱雌激素作用和潜在抗氧化作用的异黄酮成分之一。然而，关于青春期前暴露于多种内分泌干扰物后的生殖影响的研究尚属空白。在本研究中，从出生后第22天（PND22）至PND35，通过灌胃法将DEHP和GEN给予青春期前雄性斯普拉格-道利大鼠，设溶剂对照组、50 mg/kg体重（bw）/天的GEN组（G）、50、150、450 mg/kg bw/天的DEHP组（D50、D150、D450）以及它们的混合物组（G + D50、G + D150、G + D450）。在PND90时，研究了一般形态学指标（体重、肛门与生殖器距离、器官重量和器官系数）、睾丸氧化还原状态及睾丸组织学。我们的结果表明，DEHP可显著降低性器官重量、器官系数和睾丸抗氧化能力，这在很大程度上取决于DEHP的剂量。然而，GEN共同给药可通过增强睾丸抗氧化酶活性部分减轻DEHP诱导的生殖损伤，这表明GEN对青春期前暴露后DEHP诱导的雄性生殖系统损伤具有保护作用，并且GEN在治疗由其他环境内分泌干扰物引起的生殖障碍的抗氧化作用方面可能具有广阔前景。
