Saporta Samuel, Kim Jong-Joong, Willing Alison E, Fu Eugene S, Davis Cyndy D, Sanberg Paul R
Center for Aging and Brain Repair and Department of Anatomy, University of South Florida College of Medicine, Tampa, FL 33612, USA.
J Hematother Stem Cell Res. 2003 Jun;12(3):271-8. doi: 10.1089/152581603322023007.
The use of human umbilical cord blood (hUCB)--a rich source of nonembryonic or adult stem cells--has recently been reported to ameliorate behavioral consequences of stroke. In this study, we tested whether human cord blood leukocytes also ameliorate behavioral impairments of spinal cord injury. Rats were divided into five groups: (1) laminectomy (without spinal cord injury) only; (2) laminectomy + cord blood infusion; (3) spinal cord injury + cord blood infused 1 day post injury; (4) spinal cord injury + cord blood infused 5 days post injury; and (5) spinal cord injury only. Spinal cord injury was induced by compressing the spinal cord for 1 min with an aneurysm clip calibrated to a closing pressure of 55 g. Open-field behavior was assessed 1, 2, and 3 weeks after intravenous injection of prelabeled human cord blood cells. Open-field test scores of spinal cord injured rats treated with human cord blood at 5 days were significantly improved as compared to scores of rats similarly injured but treated at day 1 as well as the otherwise untreated injured group. The results suggest that cord blood stem cells are beneficial in reversing the behavioral effects of spinal cord injury, even when infused 5 days after injury. Human cord blood-derived cells were observed in injured areas, but not in noninjured areas, of rat spinal cords, and were never seen in corresponding areas of spinal cord of noninjured animals. The results are consistent with the hypothesis that cord blood-derived stem cells migrate to and participate in the healing of neurological defects caused by traumatic assault.
人脐带血（hUCB）是一种丰富的非胚胎或成体干细胞来源，最近有报道称其可改善中风的行为后果。在本研究中，我们测试了人脐带血白细胞是否也能改善脊髓损伤的行为障碍。将大鼠分为五组：（1）仅行椎板切除术（无脊髓损伤）；（2）椎板切除术+脐带血输注；（3）脊髓损伤+损伤后1天输注脐带血；（4）脊髓损伤+损伤后5天输注脐带血；（5）仅脊髓损伤。使用校准至闭合压力为55 g的动脉瘤夹压迫脊髓1分钟诱导脊髓损伤。在静脉注射预先标记的人脐带血细胞后1、2和3周评估旷场行为。与在第1天接受治疗的类似损伤大鼠以及未接受治疗的损伤组相比，在第5天接受人脐带血治疗的脊髓损伤大鼠的旷场测试得分显著提高。结果表明，即使在损伤后5天输注，脐带血干细胞也有利于逆转脊髓损伤的行为影响。在大鼠脊髓的损伤区域观察到了人脐带血来源的细胞，但在未损伤区域未观察到，且在未损伤动物脊髓的相应区域也从未见过。这些结果与脐带血来源的干细胞迁移至并参与创伤性攻击所致神经缺陷愈合的假说一致。
