Li Shihong, De Wijn Joost R, Li Jiaping, Layrolle Pierre, De Groot Klaas
IsoTis NV, Bilthoven, The Netherlands.
Tissue Eng. 2003 Jun;9(3):535-48. doi: 10.1089/107632703322066714.
Macroporous biphasic calcium phosphate (BCP) with channel-shaped pores was produced by a novel dual-phase mixing method. The processing route includes mixing water-based BCP slurry and polymethylmethacrylate resin; shaping in a mold; and polymerization, drying, pyrolyzing, and sintering. After comparison with two other commercial macroporous BCP materials, which were produced along different routes, it was found that conventional parameters such as porosity and pore size cannot describe a macroporous structure precisely enough for the application as tissue-engineering scaffold. Instead, permeability can be seen as an intrinsic and quantitative parameter to describe the macroporous structure of various scaffolds, because it is independent of sample size and fluid used in the test. Another parameter, the permeability/porosity ratio, provides an indication of the percolative efficiency per unit porous volume of a scaffold. Structural characterizations and permeability studies of other macroporous scaffold materials were also performed, and it was found that permeability could reflect a combination of five important parameters for scaffold: (1) porosity, (2) pore size and distribution, (3) interconnectivity, (4) fenestration size and distribution, and (5) orientation of pores. Finally, the implications of relating permeability with biological performances are also discussed.
通过一种新型的双相混合法制备了具有通道状孔隙的大孔双相磷酸钙（BCP）。加工路线包括将水基BCP浆料与聚甲基丙烯酸甲酯树脂混合；在模具中成型；以及聚合、干燥、热解和烧结。在与通过不同路线生产的其他两种商业大孔BCP材料进行比较后，发现诸如孔隙率和孔径等传统参数不足以精确描述用于组织工程支架应用的大孔结构。相反，渗透率可被视为描述各种支架大孔结构的固有定量参数，因为它与样品尺寸和测试中使用的流体无关。另一个参数，渗透率/孔隙率比，提供了支架每单位多孔体积的渗透效率指标。还对其他大孔支架材料进行了结构表征和渗透率研究，发现渗透率可以反映支架的五个重要参数的组合：（1）孔隙率，（2）孔径和分布，（3）连通性，（4）窗孔尺寸和分布，以及（5）孔隙取向。最后，还讨论了将渗透率与生物学性能相关联的意义。
