Kim Unkyu, Siegel Rachael, Ren Xiaodi, Gunther Cary S, Gaasterland Terry, Roeder Robert G
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8868-73. doi: 10.1073/pnas.1033108100. Epub 2003 Jul 11.
The tissue-specific transcriptional coactivator OCA-B is required for antigen-dependent B cell differentiation events, including germinal center formation. However, the identity of OCA-B target genes involved in this process is unknown. This study has used large-scale cDNA arrays to monitor changes in gene expression patterns that accompany mature B cell differentiation. B cell receptor ligation alone induces many genes involved in B cell expansion, whereas B cell receptor and helper T cell costimulation induce genes associated with B cell effector function. OCA-B expression is induced by both B cell receptor ligation alone and helper T cell costimulation, suggesting that OCA-B is involved in B cell expansion as well as B cell function. Accordingly, several genes involved in cell proliferation and signaling, such as Lck, Kcnn4, Cdc37, cyclin D3, B4galt1, and Ms4a11, have been identified as OCA-B-dependent genes. Further studies on the roles played by these genes in B cells will contribute to an understanding of B cell differentiation.
组织特异性转录共激活因子OCA - B是抗原依赖性B细胞分化事件所必需的，包括生发中心的形成。然而，参与这一过程的OCA - B靶基因的身份尚不清楚。本研究利用大规模cDNA阵列来监测伴随成熟B细胞分化的基因表达模式的变化。单独的B细胞受体连接可诱导许多参与B细胞扩增的基因，而B细胞受体和辅助性T细胞共刺激则诱导与B细胞效应功能相关的基因。单独的B细胞受体连接和辅助性T细胞共刺激均可诱导OCA - B的表达，这表明OCA - B参与B细胞扩增以及B细胞功能。因此，几个参与细胞增殖和信号传导的基因，如Lck、Kcnn4、Cdc37、细胞周期蛋白D3、B4galt1和Ms4a11，已被鉴定为OCA - B依赖性基因。对这些基因在B细胞中所起作用的进一步研究将有助于理解B细胞分化。
