Shi Xiaohong, McCaughey Conall, Elliott Richard M
Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, UK.
J Med Virol. 2003 Sep;71(1):105-9. doi: 10.1002/jmv.10446.
Seoul (SEO) viruses belong to the Hantavirus genus (family Bunyaviridae), cause the moderate form of haemorrhagic fever with renal syndrome, and have been associated with laboratory-acquired infections in many countries. To investigate the pedigree of an isolate, designated IR461, which was obtained from a laboratory-acquired infection in a UK research institute, we determined the nucleotide sequences of the small (S) and medium (M) genome segments. In addition, we determined the sequences of the S segments of two Chinese isolates (R22 and L99) and an American isolate (Tchoupitoulas [TCH]). The S segments range within 1769-1785 nucleotides in length and showed identities of >88% in nucleotide sequence and 97% in amino acid sequence to those of published S segment sequences. The M RNA segment of IR461 is 3651 nucleotides long and shows >84% identity at the nucleotide level and >98% at the amino acid level to the M segments of other SEO viruses. These data confirm that SEO viruses show the least diversity within the Hantavirus genus. Phylogenetic analyses of the sequences showed geographic clustering of the Chinese SEO viruses, and that IR461 was more closely related to SEO viruses isolated in the New World than to those from the Far East.
汉城（SEO）病毒属于汉坦病毒属（布尼亚病毒科），可引起肾综合征出血热的中度症状，并且在许多国家都与实验室感染有关。为了研究从英国一家研究机构的实验室感染中分离出的一株名为IR461的病毒的谱系，我们测定了其小（S）基因组片段和中（M）基因组片段的核苷酸序列。此外，我们还测定了两株中国分离株（R22和L99）以及一株美国分离株（丘皮图拉斯[TCH]）的S片段序列。S片段长度在1769 - 1785个核苷酸之间，其核苷酸序列与已发表的S片段序列的同一性>88%，氨基酸序列同一性为97%。IR461的M RNA片段长3651个核苷酸，其核苷酸水平与其他SEO病毒的M片段同一性>84%，氨基酸水平同一性>98%。这些数据证实，SEO病毒在汉坦病毒属中表现出最小的多样性。序列的系统发育分析表明，中国的SEO病毒存在地理聚类现象，并且IR461与在新世界分离出的SEO病毒的亲缘关系比与远东地区的病毒更为密切。
