Shi X, Liang M, Hang C, Song G, McCaughey C, Elliott R M
Institute of Virology, University of Glasgow, UK.
Virus Res. 1998 Jul;56(1):69-76. doi: 10.1016/s0168-1702(98)00065-3.
The medium (M) genome segment of hantaviruses (family Bunyaviridae) encodes the two virion glycoproteins. G1 and G2, as a precursor protein in the complementary sense RNA. We determined the nucleotide sequences of the M genome segments of three Chinese hantavirus isolates, a Hantaan-type (HTN) virus designated A9 and two Seoul-type (SEO) viruses designated L99 and HB55, and compared them to those of other HTN or SEO viruses isolated in Eastern Asia. The M segment of A9 is 3616 nucleotides in length and shows 99.5% identity at the nucleotide level and 99.1% identity at the amino acid level to that of the Chinese HTN isolate HV114. The M segments of L99 and HB55 are 3652 nucleotides in length, one nucleotide longer than the M segments of other sequenced SEO isolates such as SEO 80-39, SR-11, and Biken-1. The Chinese SEO isolates showed 95% nucleotide sequence identity and 99% amino acid sequence identity to SEO 80-39. We also sequenced a 736 nucleotides region of the M genome segment of another Chinese SEO isolate, R22, which revealed errors in the published data. Phylogenetic analysis of the available sequences indicated that both the Chinese HTN- and SEO-type viruses form lineages distinct from those of the isolates from other parts of Eastern Asia.
汉坦病毒（布尼亚病毒科）的中（M）基因组片段编码两种病毒粒子糖蛋白G1和G2，以互补义RNA形式作为前体蛋白。我们测定了三株中国汉坦病毒分离株M基因组片段的核苷酸序列，一株汉滩型（HTN）病毒命名为A9，两株汉城型（SEO）病毒命名为L99和HB55，并将它们与在东亚分离的其他HTN或SEO病毒的序列进行比较。A9的M片段长度为3616个核苷酸，在核苷酸水平上与中国HTN分离株HV114的序列一致性为99.5%，在氨基酸水平上为99.1%。L99和HB55的M片段长度为3652个核苷酸，比其他已测序的SEO分离株如SEO 80-39、SR-11和Biken-1的M片段长一个核苷酸。中国的SEO分离株与SEO 80-39在核苷酸序列上的一致性为95%，在氨基酸序列上为99%。我们还对另一株中国SEO分离株R22的M基因组片段的736个核苷酸区域进行了测序，结果显示已发表的数据存在错误。对现有序列的系统发育分析表明，中国的HTN型和SEO型病毒均形成了与东亚其他地区分离株不同的谱系。
