来自粳稻的28000多个cDNA克隆的收集、定位和注释。

Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice.

作者信息

Kikuchi Shoshi, Satoh Kouji, Nagata Toshifumi, Kawagashira Nobuyuki, Doi Koji, Kishimoto Naoki, Yazaki Junshi, Ishikawa Masahiro, Yamada Hitomi, Ooka Hisako, Hotta Isamu, Kojima Keiichi, Namiki Takahiro, Ohneda Eisuke, Yahagi Wataru, Suzuki Kohji, Li Chao Jie, Ohtsuki Kenji, Shishiki Toru, Otomo Yasuhiro, Murakami Kazuo, Iida Yoshiharu, Sugano Sumio, Fujimura Tatsuto, Suzuki Yutaka, Tsunoda Yuki, Kurosaki Takashi, Kodama Takeko, Masuda Hiromi, Kobayashi Michie, Xie Quihong, Lu Min, Narikawa Ryuya, Sugiyama Akio, Mizuno Kouichi, Yokomizo Satoko, Niikura Junko, Ikeda Rieko, Ishibiki Junya, Kawamata Midori, Yoshimura Akemi, Miura Junichirou, Kusumegi Takahiro, Oka Mitsuru, Ryu Risa, Ueda Mariko, Matsubara Kenichi, Kawai Jun, Carninci Piero, Adachi Jun, Aizawa Katsunori, Arakawa Takahiro, Fukuda Shiro, Hara Ayako, Hashizume Wataru, Hayatsu Norihito, Imotani Koichi, Ishii Yoshiyuki, Itoh Masayoshi, Kagawa Ikuko, Kondo Shinji, Konno Hideaki, Miyazaki Ai, Osato Naoki, Ota Yoshimi, Saito Rintaro, Sasaki Daisuke, Sato Kenjiro, Shibata Kazuhiro, Shinagawa Akira, Shiraki Toshiyuki, Yoshino Masayasu, Hayashizaki Yoshihide, Yasunishi Ayako

机构信息

Department of Molecular Biology, National Institute of Agrobiological Sciences, 2-1-2 Kannon-dai, Tsukuba, Ibaraki 305-8602, Japan.

出版信息

Science. 2003 Jul 18;301(5631):376-9. doi: 10.1126/science.1081288.

DOI:10.1126/science.1081288

PMID:12869764

Abstract

We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription units in the rice genome. Protein informatics analysis against the InterPro database revealed the existence of proteins presented in rice but not in Arabidopsis. Sixty-four percent of our cDNAs are homologous to Arabidopsis proteins.

摘要

我们从粳稻品种日本晴中收集并对28469个全长互补DNA克隆进行了全序列测定。通过对公开可用序列数据的同源性搜索，我们为21596个克隆（75.86%）暂定了蛋白质功能。将这些cDNA克隆定位到基因组DNA上表明，水稻基因组中有19000至20500个转录单元。针对InterPro数据库进行的蛋白质信息学分析揭示了水稻中存在但拟南芥中不存在的蛋白质。我们64%的cDNA与拟南芥蛋白质同源。