Naglik Julian R, Rodgers Catherine A, Shirlaw Penelope J, Dobbie Jennifer L, Fernandes-Naglik Lynette L, Greenspan Deborah, Agabian Nina, Challacombe Stephen J
Department of Oral Medicine, Pathology, and Immunology, Guy's, King's, and St. Thomas' Dental Institute, Kings College London, London, United Kingdom.
J Infect Dis. 2003 Aug 1;188(3):469-79. doi: 10.1086/376536. Epub 2003 Jul 14.
The in vivo expression of Candida albicans secreted aspartyl proteinase (SAP1-SAP8) and phospholipase B (PLB1 and PLB2) genes was analyzed in 137 human subjects with oral and vaginal candidiasis or carriage. Total RNA was isolated from whole unstimulated saliva or vaginal swabs, and the expression of SAP1-8 and PLB1-2 was evaluated by reverse-transcriptase polymerase chain reaction using specific primer sets. A spectrum of SAP gene expression profiles was obtained from different C. albicans strains during symptomatic disease and asymptomatic carriage. SAP2 and SAP5 were the most common genes expressed during both infection and carriage. SAP1, SAP3, SAP4, SAP7, SAP8, and PLB1 expression was correlated with oral disease, whereas SAP1, SAP3, and SAP6-SAP8 expression was correlated with vaginal disease. Furthermore, SAP1, SAP3, and SAP8 were preferentially expressed in vaginal, rather than oral, infections. This study demonstrates the differential expression of the hydrolytic enzyme genes in humans and correlates the expression of specific Candida species virulence genes with active disease and anatomical location.
对137名患有口腔和阴道念珠菌病或念珠菌携带的人类受试者，分析了白色念珠菌分泌天冬氨酸蛋白酶（SAP1 - SAP8）和磷脂酶B（PLB1和PLB2）基因的体内表达情况。从未经刺激的全唾液或阴道拭子中分离总RNA，使用特异性引物对通过逆转录聚合酶链反应评估SAP1 - 8和PLB1 - 2的表达。在有症状疾病和无症状携带期间，从不同白色念珠菌菌株获得了一系列SAP基因表达谱。SAP2和SAP5是感染和携带期间最常表达的基因。SAP1、SAP3、SAP4、SAP7、SAP8和PLB1的表达与口腔疾病相关，而SAP1、SAP3和SAP6 - SAP8的表达与阴道疾病相关。此外，SAP1、SAP3和SAP8在阴道感染而非口腔感染中优先表达。本研究证明了水解酶基因在人类中的差异表达，并将特定念珠菌属毒力基因的表达与活动性疾病和解剖位置相关联。
