Scott T W, Amerasinghe P H, Morrison A C, Lorenz L H, Clark G G, Strickman D, Kittayapong P, Edman J D
Department of Entomology, University of California, Davis 95616, USA.
J Med Entomol. 2000 Jan;37(1):89-101. doi: 10.1603/0022-2585-37.1.89.
We used a histologic technique to study multiple blood feeding in a single gonotrophic cycle by engorged Aedes aegypti (L.) that were collected weekly for 2 yr from houses in a rural village in Thailand (n = 1,891) and a residential section of San Juan, Puerto Rico (n = 1,675). Overall, mosquitoes from Thailand contained significantly more multiple meals (n = 1,300, 42% double meals, 5% triple meals) than mosquitoes collected in Puerto Rico (n = 1,156, 32% double meals, 2% triple meals). The portion of specimens for which frequency of feeding could not be determined was 31% at both sites. We estimated that on average Ae. aegypti take 0.76 and 0.63 human blood meals per day in Thailand and Puerto Rico, respectively. However, frequency of multiple feeding varied among houses and, in Puerto Rico, the neighborhoods from which mosquitoes were collected. In Thailand 65% of the mosquitoes fed twice on the same day, whereas in Puerto Rico 57% took multiple meals separated by > or = 1 d. At both sites, the majority of engorged specimens were collected inside houses (Thailand 86%, Puerto Rico 95%). The number of blood meals detected was independent of where mosquitoes were collected (inside versus outside of the house) at both sites and the time of day collections were made in Puerto Rico. Feeding rates were slightly higher for mosquitoes collected in the afternoon in Thailand. Temperatures were significantly higher and mosquitoes significantly smaller in Thailand than in Puerto Rico. At both sites female size was negatively associated with temperature. Rates of multiple feeding were associated positively with temperature and negatively with mosquito size in Thailand, but not in Puerto Rico. Multiple feeding during a single gonotrophic cycle is a regular part of Ae. aegypti biology, can vary geographically and under different climate conditions, and may be associated with variation in patterns of dengue virus transmission.
我们采用一种组织学技术，对泰国一个乡村房屋（n = 1891）和波多黎各圣胡安一个居民区（n = 1675）中每周采集的饱血埃及伊蚊（L.）在单个生殖营养周期内的多次吸血情况进行了研究，为期2年。总体而言，来自泰国的蚊子所含的多次吸血情况（n = 1300，42%为二次吸血，5%为三次吸血）显著多于在波多黎各采集的蚊子（n = 1156，32%为二次吸血，2%为三次吸血）。在两个地点，无法确定吸血频率的标本比例均为31%。我们估计，在泰国和波多黎各，埃及伊蚊平均每天分别吸食0.76次和0.63次人血。然而，多次吸血的频率在不同房屋之间存在差异，在波多黎各，还因采集蚊子的社区不同而有所差异。在泰国，65%的蚊子在同一天内吸食两次血，而在波多黎各，57%的蚊子多次吸血的间隔时间≥1天。在两个地点，大多数饱血标本都是在房屋内采集到的（泰国为86%，波多黎各为95%）。在两个地点，检测到的吸血次数与蚊子的采集地点（房屋内还是房屋外）无关，在波多黎各，与采集时间也无关。在泰国，下午采集的蚊子吸血率略高。泰国的温度显著高于波多黎各，且蚊子明显更小。在两个地点，雌蚊大小均与温度呈负相关。在泰国，多次吸血率与温度呈正相关，与蚊子大小呈负相关，但在波多黎各并非如此。在单个生殖营养周期内的多次吸血是埃及伊蚊生物学的一个常规组成部分，在地理上和不同气候条件下会有所不同，并且可能与登革病毒传播模式的变化有关。
