Chen K, Feng H, Zhang M, Wang X
School of Life Sciences, Lanzhou University, Lanzhou 730 000, Peoples Republic of China.
Folia Microbiol (Praha). 2003;48(3):389-93. doi: 10.1007/BF02931372.
The effect of ultraviolet-B radiation (UV-B; 280-320 nm) on induction of nitric oxide was estimated in the suspensions of green alga Chlorella pyrenoidosa with or without the NO scavenger N-acetyl-L-cysteine, and reductants such as 1,4-dithiothreitol, glutathione (reduced form), and ascorbic acid. Exogenously added sodium nitroprusside (NO donor), glutathione, 1,4-dithiothreitol, and ascorbic acid were able to prevent chlorophyll loss mediated by UV-B. Addition of NO to algal suspensions irradiated by UV-B increased the activity of catalase and superoxide dismutase but lowered the activity of phenylalanine ammonia-lyase. UV-B thus appears to be a strong inducer of NO production, exogenously added NO and reductants protecting the green alga against UV-B-induced oxidative damage.
在有或没有一氧化氮清除剂N-乙酰-L-半胱氨酸以及还原剂（如1,4-二硫苏糖醇、还原型谷胱甘肽和抗坏血酸）存在的情况下，在绿藻小球藻的悬浮液中评估了紫外线B辐射（UV-B；280 - 320纳米）对一氧化氮诱导的影响。外源添加的硝普钠（一氧化氮供体）、谷胱甘肽、1,4-二硫苏糖醇和抗坏血酸能够防止UV-B介导的叶绿素损失。向受UV-B照射的藻类悬浮液中添加一氧化氮可提高过氧化氢酶和超氧化物歧化酶的活性，但会降低苯丙氨酸解氨酶的活性。因此，UV-B似乎是一氧化氮产生的强诱导剂，外源添加的一氧化氮和还原剂可保护绿藻免受UV-B诱导的氧化损伤。
