生长抑素受体在中间型和恶性软组织肿瘤中的体外及体内表达
In vitro and in vivo expression of somatostatin receptors in intermediate and malignant soft tissue tumors.
作者信息
Florio Tullio, Montella Liliana, Corsaro Alessandro, De Chiara Annarosaria, Apice Gaetano, Fazioli Flavio, Lastoria Secondo, Schettini Gennaro, Palmieri Giovannella
机构信息
National Institute for Cancer Research, Advanced Biotechnology Center, Largo Rosanna Benzi 10, 16132 Genova, Italy.
出版信息
Anticancer Res. 2003 May-Jun;23(3B):2465-71.
BACKGROUND
Previous studies showed that the subtype 2 of somatostatin receptors (sstr2) is generally expressed by soft tissue sarcomas.
MATERIALS AND METHODS
Using reverse transcription-polymerase chain reaction (RT-PCR), the present study evaluated the expression of sstr mRNA in 31 human intermediate and malignant soft tissue tumors. In 8 patients somatostatin receptor scintigraphy was performed to detect soft tissue tumor sites and visualize in vivo sstr expression.
RESULTS
Somatostatin receptors were identified in 84% of the tumors analyzed by RT-PCR analysis. sstr1 and sstr2 were the receptors most frequently detected. Somatostatin receptor scintigraphy was able to identify primary and/or metastatic sites in 7 out of 8 patients, with an overall sensitivity per site of 87%. Concordant results were found between in vitro and in vivo techniques.
CONCLUSION
The wide expression of sstrs in soft tissue tumors suggest a relevant role for these receptors in diagnosis and may have therapeutic applications.
背景
先前的研究表明，生长抑素受体2型（sstr2）通常在软组织肉瘤中表达。
材料与方法
本研究采用逆转录聚合酶链反应（RT-PCR）评估了31例人类中、恶性软组织肿瘤中sstr mRNA的表达。对8例患者进行了生长抑素受体闪烁显像，以检测软组织肿瘤部位并在体内显示sstr的表达情况。
结果
通过RT-PCR分析，在84%的肿瘤中检测到了生长抑素受体。sstr1和sstr2是最常检测到的受体。生长抑素受体闪烁显像能够在8例患者中的7例中识别出原发和/或转移部位，每个部位的总体敏感性为87%。体外和体内技术的结果一致。
结论
sstrs在软组织肿瘤中的广泛表达表明这些受体在诊断中具有重要作用，并且可能具有治疗应用价值。
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