Steinbach Günter, Methner Ullrich, Meyer Horst
Bundesforschungsanstalt für Viruskrankheiten der Tiere, Standort Jena.
Berl Munch Tierarztl Wochenschr. 2003 Jul-Aug;116(7-8):274-80.
Using the close linear regression between the logarithm of the dilution degree of a sample and the logarithm of the extinction measured in an ELISA both the relative concentrations of immunoglobulines of the isotypes IgG, IgM and IgA and of the LPS antibodies against S. Typhimurium of the different isotypes in blood sera and meat juice of 15 slaughtered pigs were detected and compared. Furthermore the total concentration of antibodies against LPS of S. Typhimurium according to the "meat juice ELISA" were compared. Distribution of immunoglobulines between serum and meat juice revealed individual differences between the animals as well as between the different immunoglobulin-isotypes. Within the same isotype the ratio of the concentrations of anti-LPS Salmonella Typhimurium antibodies between serum and meat juice was significantly closer than relating the whole of immunoglobulines of the referred isotype. In order to detect pig herds with a high level of Salmonella exposure a comparison of the 1:30 diluted meat juice samples with the 1:400 diluted blood sera is justified, however, for detailed epidemiological or scientific studies there is a need to consider the existing differences between the immunoglobuline-isotypes as well as between the specificity of antibodies and of total immunoglobulines. While the concentration of Salmonella antibodies of the isotypes IgG1, IgG2 and IgA showed a clear and statistically significant correlation between both one below the other and with the total amount of Salmonella antibodies, this connection could not be established for the total amount of immunoglobulines of different isotypes and the IgM-antibodies.
利用样品稀释度的对数与酶联免疫吸附测定（ELISA）中测得的吸光度对数之间的紧密线性回归关系，检测并比较了15头屠宰猪血清和肉汁中免疫球蛋白IgG、IgM和IgA同型以及不同同型抗鼠伤寒沙门氏菌脂多糖（LPS）抗体的相对浓度。此外，还比较了根据“肉汁ELISA”法测得的抗鼠伤寒沙门氏菌LPS抗体的总浓度。免疫球蛋白在血清和肉汁之间的分布显示出动物个体之间以及不同免疫球蛋白同型之间的差异。在同一同型内，血清和肉汁中抗鼠伤寒沙门氏菌LPS抗体浓度的比值明显比该同型的整个免疫球蛋白的比值更接近。为了检测沙门氏菌暴露水平高的猪群，将1:30稀释的肉汁样品与1:400稀释的血清样品进行比较是合理的，然而，对于详细的流行病学或科学研究，有必要考虑免疫球蛋白同型之间以及抗体特异性与总免疫球蛋白之间存在的差异。虽然IgG1、IgG2和IgA同型的沙门氏菌抗体浓度在彼此之间以及与沙门氏菌抗体总量之间显示出明显且具有统计学意义的相关性，但不同同型免疫球蛋白总量与IgM抗体之间无法建立这种联系。
