Bomba A, Kastel R, Gancarclková S, Nemcová R, Herich R, Cizek M
Research Institute of Experimental Veterinary Medicine, Kosice, Slovak Republic.
Berl Munch Tierarztl Wochenschr. 1996 Nov-Dec;109(11-12):428-30.
The effect of the inoculation of three Lactobacillus plantarum strains upon lactic, acetic, acetoacetic and propionic acid levels in the mucosal film (F) and the jejunal and ileal contents (O) has been investigated in gnotobiotic pigs. In the jejunum of the inoculated animals, the mucosal film revealed significantly increased levels of lactic, propionic and acetoacetic acids when compared to the contents (25.3 vs. 10.8 mmol.l-1, 18.5 vs. 5 mmol.l-1 and 29.7 vs. 11.2 mmol.l-1, respectively) as well as insignificantly increased acetic acid levels (11.0 vs. 5.8 mmol.l-1). In the ileum of gnotobiotic pigs, propionic acid levels of the mucosal film were significantly higher than those of the contents (21.2 vs. 9.5 mmol.l-1, p < 0.05). In comparison to the contents, the increased lactic, acetic and acetoacetic acid levels in the film proved to be insignificant. The above results suggest that the significantly increased levels of the Lactobacilli-produced organic acids may present an efficient barrier inhibiting the adherence of digestive tract pathogens to the intestinal mucosa.
在无菌猪中研究了接种三种植物乳杆菌菌株对粘膜层（F）以及空肠和回肠内容物（O）中乳酸、乙酸、乙酰乙酸和丙酸水平的影响。在接种动物的空肠中，与内容物相比，粘膜层中乳酸、丙酸和乙酰乙酸水平显著升高（分别为25.3对10.8 mmol·l-1、18.5对5 mmol·l-1和29.7对11.2 mmol·l-1），乙酸水平升高不显著（11.0对5.8 mmol·l-1）。在无菌猪的回肠中，粘膜层的丙酸水平显著高于内容物（21.2对9.5 mmol·l-1，p<0.05）。与内容物相比，粘膜层中乳酸、乙酸和乙酰乙酸水平的升高不显著。上述结果表明，乳酸杆菌产生的有机酸水平显著升高可能构成一道有效屏障，抑制消化道病原体粘附于肠粘膜。
