Gomes I, Gomes J, Steiner W
Bangladesh Jute Research Institute, Manik Mian Avenue, 1207 Dhaka, Bangladesh.
Bioresour Technol. 2003 Nov;90(2):207-14. doi: 10.1016/s0960-8524(03)00110-x.
Five strains of the extreme thermophilic Rhodothermus marinus were screened for the production of amylolytic and pullulytic activities. The culture medium for the selected strain, R. marinus ITI 990, was optimized using central composite designs for enhanced enzyme production. The optimized medium containing 1.5 gl(-1) of maltose and 8.3 gl(-1) of yeast extract yielded amylase, pullulanase and alpha-glucosidase activities of 45, 33 and 2.1 nkatml(-1), respectively. Among the various carbon sources tested, maltose was most effective for the formation of these enzymes, followed by soluble maize starch, glycogen and pullulan. The crude amylase and pullulanase showed maximum activities at pH 6.5-7.0, and 85 and 80 degrees C, respectively. At 85 degrees C amylase and pullulanase had half lives of 3 h and 30 min, respectively.
筛选了五株嗜热栖热放线菌（Rhodothermus marinus）以检测其淀粉酶和支链淀粉酶活性。使用中心复合设计对所选菌株嗜热栖热放线菌ITI 990的培养基进行了优化以提高酶产量。含有1.5 g l⁻¹麦芽糖和8.3 g l⁻¹酵母提取物的优化培养基分别产生了45、33和2.1 nkat ml⁻¹的淀粉酶、支链淀粉酶和α-葡萄糖苷酶活性。在测试的各种碳源中麦芽糖对这些酶的形成最有效其次是可溶性玉米淀粉、糖原和支链淀粉。粗淀粉酶和支链淀粉酶分别在pH 6.5 - 7.0以及85和80℃时表现出最大活性。在85℃时淀粉酶和支链淀粉酶的半衰期分别为3小时和30分钟。
