Kim J H, Kim S C
Center for Advanced Functional Polymers, Korea Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yusong-gu, Taejon 305-701, South Korea.
J Biomater Sci Polym Ed. 2003;14(6):601-14. doi: 10.1163/15685620360674281.
When hydrophilic/hydrophobic polymers have a microdomain structure, platelet adhesion and activation are effectively suppressed by prohibition of the excessive assembly of glycoproteins and adenosine triphosphate (ATP) consumption of the platelets on the surface. In this study, poly(ethylene oxide)-grafted hydrophilic polyurethane (PU)/hydrophobic polystyrene (PS) interpenetrating polymer networks (IPNs) were synthesized by varying the synthesis temperature to control the phase separation and the microdomain surface structure, and the effect of the degree of phase separation on the in vitro blood compatibility. The size of the dispersed PS-rich domains in the PU-rich matrix decreased, and the hydrophilicity also decreased as the synthesis temperature of the PS network during the IPN synthesis was decreased, as the phase separation was suppressed during the synthesis. The amount of the adsorbed bovine plasma fibrinogens (BPF) on the PEO-grafted PU/PS IPNs decreased as the synthesis temperature was decreased, and the in vitro adhesion of the platelets was also suppressed on the PEO-grafted PU/PS IPNs prepared at lower temperature. The microdomain structure on the surface affected the adhesion and the activation of the adhered platelets, and the suppression of the phase separation resulted in the decrease of the domain size, which also enhanced the blood compatibility of the PEO-grafted PU/PS IPNs.
当亲水性/疏水性聚合物具有微区结构时，通过抑制糖蛋白的过度聚集以及血小板在表面的三磷酸腺苷（ATP）消耗，可有效抑制血小板的黏附和活化。在本研究中，通过改变合成温度来控制相分离和微区表面结构，合成了聚环氧乙烷接枝的亲水性聚氨酯（PU）/疏水性聚苯乙烯（PS）互穿聚合物网络（IPN），并研究了相分离程度对体外血液相容性的影响。随着IPN合成过程中PS网络合成温度的降低，富PU基质中富PS分散域的尺寸减小，亲水性也降低，因为合成过程中的相分离受到抑制。随着合成温度的降低，聚环氧乙烷接枝的PU/PS IPN上吸附的牛血浆纤维蛋白原（BPF）量减少，在较低温度下制备的聚环氧乙烷接枝的PU/PS IPN上，血小板的体外黏附也受到抑制。表面的微区结构影响黏附血小板的黏附和活化，相分离的抑制导致域尺寸减小，这也增强了聚环氧乙烷接枝的PU/PS IPN的血液相容性。
