Dittrich Sabine, Schwöbel Babett, Jordan Sabine, Vanisaveth Vienxay, Rattanaxay Phetsouvanh, Christophel Eva M, Phompida Samlane, Jelinek Tomas
Department of Infectious Diseases and Tropical Medicine, University of Munich, Munich, Germany.
Malar J. 2003 Jul 22;2:23. doi: 10.1186/1475-2875-2-23.
The erythrocyte binding antigen 175 (EBA-175) is a 175 kDa antigen of Plasmodium falciparum and plays a major role in erythrocyte recognition by the parasite. The antigen is also supposed to be partly responsible for the invasion of erythrocytes by merozoites. EBA-175 has been sequenced from the FCR-3 and CAMP strains of P. falciparum. The sequences were identical in most parts of the gene. Differences were apparent in a 423 bp segment in the FCR-3 strain, the F-Fragment, that is not found in the CAMP-strain and a 342 bp segment, the C-Fragment, which is present in the CAMP-strain but not in the FCR-3-strain. The aim of this study was to assess the distribution of the two EBA-175-alleles in the Lao PDR.
MATERIALS & METHODS: Altogether, 240 blood-samples were collected in two areas of the country: Attapeu in the south and Lung Namtha in the north. Subsequently, the material was scanned for the F-and C-fragments.
In the whole study population, 52% carried the F-fragment, and 41% the C-fragment while seven percent of the patients were infected with at least two parasite strains and showed both alleles.
Distribution of the alleles showed significant differences between the north and the south province. Reasons for this include possible importation of different parasite strains from neighbouring countries.
红细胞结合抗原175（EBA - 175）是恶性疟原虫的一种175 kDa抗原，在寄生虫识别红细胞过程中起主要作用。该抗原也被认为部分负责裂殖子对红细胞的入侵。已对恶性疟原虫的FCR - 3和CAMP菌株的EBA - 175进行了测序。基因的大部分区域序列相同。在FCR - 3菌株的一个423 bp片段（F片段）中存在差异，CAMP菌株中未发现该片段；在CAMP菌株中存在一个342 bp片段（C片段），而FCR - 3菌株中没有。本研究的目的是评估老挝人民民主共和国两种EBA - 175等位基因的分布情况。
在该国两个地区共采集了240份血样：南部的阿塔佩县和北部的琅南塔省。随后，对样本进行F片段和C片段的检测。
在整个研究人群中，52%携带F片段，41%携带C片段，7%的患者感染了至少两种寄生虫菌株并显示出两种等位基因。
等位基因的分布在北部和南部省份之间存在显著差异。原因可能包括从邻国输入不同的寄生虫菌株。
