Sawasaki T, Hasegawa Y, Tsuchimochi M, Kasahara Y, Endo Y
Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama 790-8577, Japan.
Nucleic Acids Symp Ser. 2000(44):9-10. doi: 10.1093/nass/44.1.9.
Using the expression vector, pEU, which we have constructed, highly efficient in vitro protein synthesis can be achieved: The system works for 150 hours and without further template addition once the reaction has started, yielding 5 mg of enzymatically active protein in a 1 ml reaction.
使用我们构建的表达载体pEU，可以实现高效的体外蛋白质合成：该系统一旦反应开始就能工作150小时，且无需进一步添加模板，在1毫升反应中可产生5毫克具有酶活性的蛋白质。
