从猪粪便中分离的乳酸菌产生罗伊氏菌素及益生菌特性评估。
Reuterin production by lactobacilli isolated from pig faeces and evaluation of probiotic traits.
作者信息
Rodríguez E, Arqués J L, Rodríguez R, Nuñez M, Medina M
机构信息
Dpto. Tecnología de Alimentos, INIA, Carretera de La Coruña, Madrid, Spain.
出版信息
Lett Appl Microbiol. 2003;37(3):259-63. doi: 10.1046/j.1472-765x.2003.01390.x.
AIMS
To determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria.
METHODS AND RESULTS
Twenty-eight of 165 lactobacilli isolates produced reuterin in the presence of glycerol. Six isolates yielding high levels of reuterin with respect to type strain Lactobacillus reuteri CECT 925T were identified as Lact. reuteri. They were able to survive at pH 3 and subsequent exposure to cholic acid or oxgall, and presented bile salt hydrolase and bacteriocin-like activities.
CONCLUSIONS
Reuterin production is a frequently found trait among lactobacilli isolated from pig faeces. Selected Lact. reuteri isolates were able to survive at conditions likely to be encountered throughout the gastrointestinal tract.
SIGNIFICANCE AND IMPACT OF THE STUDY
High yields of reuterin may be obtained from selected isolates of Lact. reuteri. Probiotic characteristics of isolates studied in the present work suggest their application in food and feed.
目的
确定从猪粪便中分离出的乳酸杆菌产生罗伊氏菌素的情况，并评估它们作为益生菌的潜力。
方法与结果
165株乳酸杆菌分离株中有28株在甘油存在的情况下产生罗伊氏菌素。相对于罗伊氏乳杆菌CECT 925T模式菌株，6株产生高水平罗伊氏菌素的分离株被鉴定为罗伊氏乳杆菌。它们能够在pH 3以及随后暴露于胆酸或牛胆汁的条件下存活，并表现出胆汁盐水解酶和类细菌素活性。
结论
从猪粪便中分离出的乳酸杆菌中，产生罗伊氏菌素是一种常见特征。筛选出的罗伊氏乳杆菌分离株能够在整个胃肠道可能遇到的条件下存活。
研究的意义和影响
从筛选出的罗伊氏乳杆菌分离株中可获得高产的罗伊氏菌素。本研究中分离株的益生菌特性表明它们可应用于食品和饲料中。
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