罗伊氏乳杆菌产生的生长抑制剂罗伊氏菌素的生产与分离。
Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri.
作者信息
Talarico T L, Casas I A, Chung T C, Dobrogosz W J
机构信息
Department of Microbiology, North Carolina State University, Raleigh 27695.
出版信息
Antimicrob Agents Chemother. 1988 Dec;32(12):1854-8. doi: 10.1128/AAC.32.12.1854.
Abstract
Lactobacillus reuteri is a prominent member of the Lactobacillus population in the gastrointestinal ecosystem of humans, poultry, swine, and other animals. Reuterin is a newly discovered, broad-spectrum antimicrobial substance produced by this species during fermentation of glycerol. In this report, we describe procedures for (i) producing reuterin in sufficient amounts to isolate from a fermentation mixture and (ii) isolating this substance by high-performance liquid chromatography. By using uniformly labeled [14C]glycerol, reuterin was identified as a product of glycerol fermentation associated with the production of beta-hydroxypropionic acid and trimethylene glycol.
摘要
罗伊氏乳杆菌是人类、家禽、猪和其他动物胃肠道生态系统中乳酸菌群体的重要成员。罗伊氏菌素是该菌种在甘油发酵过程中产生的一种新发现的广谱抗菌物质。在本报告中，我们描述了以下步骤：（i）生产足够量的罗伊氏菌素以从发酵混合物中分离出来；（ii）通过高效液相色谱法分离该物质。通过使用均匀标记的[14C]甘油，罗伊氏菌素被鉴定为与β-羟基丙酸和三亚甲基二醇产生相关的甘油发酵产物。
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