20个SARS病毒基因组序列的突变分析：复制酶开放阅读框1b和刺突基因中负选择的证据
Mutation analysis of 20 SARS virus genome sequences: evidence for negative selection in replicase ORF1b and spike gene.
作者信息
Hu Lan-Dian, Zheng Guang-Yong, Jiang Hai-Song, Xia Yu, Zhang Yi, Kong Xiang-Yin
机构信息
Health Science Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Second Medical University, Shanghai 200025, China.
出版信息
Acta Pharmacol Sin. 2003 Aug;24(8):741-5.
AIM
Recently, more SARS-CoV virus genome sequences are released to the GenBank database. The aim of this study is to reveal the evolution forces of SARS-CoV virus by analyzing the nucleotide mutations in these sequences.
METHODS
We obtained 20 SARS-CoV virus genome sequences from NCBI database, and calculated the ratio of non-synonymous nucleotide substitution per non-synonymous site (Ka) and synonymous nucleotide substitution per synonymous site (Ks) for SARS-CoV virus genes.
RESULTS
The Ka/Ks ratios for replicase polyprotein ORF1a, ORF1b, and spike protein gene are 1.09 (P=0.6501), 0.38 (P=0.0074), 0.65 (P=0.0685) respectively.
CONCLUSION
SARS-CoV virus replicase polyprotein ORF1b is undergoing negative selection; negative selection force is also probably operating on spike protein gene. These results provide basis for future developing a new drug and vaccine against SARS.
目的
近期，更多严重急性呼吸综合征冠状病毒（SARS-CoV）病毒基因组序列被提交至GenBank数据库。本研究旨在通过分析这些序列中的核苷酸突变来揭示SARS-CoV病毒的进化驱动力。
方法
我们从NCBI数据库获取了20条SARS-CoV病毒基因组序列，并计算了SARS-CoV病毒基因中每个非同义位点的非同义核苷酸替代率（Ka）和每个同义位点的同义核苷酸替代率（Ks）。
结果
复制酶多聚蛋白ORF1a、ORF1b和刺突蛋白基因的Ka/Ks比值分别为1.09（P = 0.6501）、0.38（P = 0.0074）、0.65（P = 0.0685）。
结论
SARS-CoV病毒复制酶多聚蛋白ORF1b正经历负选择；负选择力可能也作用于刺突蛋白基因。这些结果为未来开发抗SARS的新药和疫苗提供了依据。
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