Ren Wuze, Li Wendong, Yu Meng, Hao Pei, Zhang Yuan, Zhou Peng, Zhang Shuyi, Zhao Guoping, Zhong Yang, Wang Shengyue, Wang Lin-Fa, Shi Zhengli
Graduate School of CAS, Beijing 100039, China.
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan, Hubei 430071, China.
J Gen Virol. 2006 Nov;87(Pt 11):3355-3359. doi: 10.1099/vir.0.82220-0.
Bats were recently identified as natural reservoirs of SARS-like coronavirus (SL-CoV) or SARS coronavirus-like virus. These viruses, together with SARS coronaviruses (SARS-CoV) isolated from human and palm civet, form a distinctive cluster within the group 2 coronaviruses of the genus Coronavirus, tentatively named group 2b (G2b). In this study, complete genome sequences of two additional group 2b coronaviruses (G2b-CoVs) were determined from horseshoe bat Rhinolophus ferrumequinum (G2b-CoV Rf1) and Rhinolophus macrotis (G2b-CoV Rm1). The bat G2b-CoV isolates have an identical genome organization and share an overall genome sequence identity of 88-92 % among themselves and between them and the human/civet isolates. The most variable regions are located in the genes encoding nsp3, ORF3a, spike protein and ORF8 when bat and human/civet G2b-CoV isolates are compared. Genetic analysis demonstrated that a diverse G2b-CoV population exists in the bat habitat and has evolved from a common ancestor of SARS-CoV.
蝙蝠最近被确定为严重急性呼吸综合征样冠状病毒（SL-CoV）或严重急性呼吸综合征冠状病毒样病毒的天然宿主。这些病毒与从人类和果子狸中分离出的严重急性呼吸综合征冠状病毒（SARS-CoV）一起，在冠状病毒属的2组冠状病毒中形成了一个独特的簇，暂命名为2b组（G2b）。在本研究中，从中华菊头蝠（G2b-CoV Rf1）和大耳菊头蝠（G2b-CoV Rm1）中确定了另外两种2b组冠状病毒（G2b-CoVs）的完整基因组序列。蝙蝠G2b-CoV分离株具有相同的基因组结构，它们之间以及与人类/果子狸分离株之间的总体基因组序列同一性为88-92%。当比较蝙蝠和人类/果子狸G2b-CoV分离株时，最可变的区域位于编码nsp3、ORF3a、刺突蛋白和ORF8的基因中。遗传分析表明，在蝙蝠栖息地存在多种G2b-CoV群体，并且它们是从SARS-CoV的共同祖先进化而来的。
