Bourlev Vladimir, Pavlovitch Stanislav, Stygar Denis, Volkov Nikolai, Lindblom Bo, Olovsson Matts
Research Centre of Obstetrics, Gynaecology and Perinatology, Russian Academy of Medical Sciences, Moscow, Russian Federation.
Gynecol Obstet Invest. 2003;55(4):199-204. doi: 10.1159/000072074.
To determine whether there are differences in proliferative and apoptotic activity and in expression of sex steroid receptors between central and peripheral parts of human uterine leiomyomas in different menstrual cycle phases.
Biopsy specimens of the myometrium and peripheral and central parts of uterine leiomyomas were obtained from 15 women in the proliferative phase and 8 women in the secretory phase. Mitotic cells were detected by immunohistochemical staining for Ki67. Apoptotic cells were detected by the TUNEL method. Mitotic and apoptotic indexes were calculated. Tissue levels of oestrogen and progesterone receptors were measured using a commercial monoclonal receptor enzyme immunoassay kit.
During the secretory phase the mitotic index was significantly higher in the peripheral than in the central parts of the leiomyomas. During the proliferative phase the apoptotic index was significantly higher in the peripheral compared with the central parts. These differences were not reflected by differences in receptor expression.
The results of this study suggest that the growth of a human uterine leiomyoma mainly occurs in the peripheral parts of the tumour, during the secretory phase of the menstrual cycle. The findings emphasise the importance of being consistent when taking samples for research work.
确定在不同月经周期阶段，人类子宫平滑肌瘤的中央和外周部分在增殖和凋亡活性以及性类固醇受体表达方面是否存在差异。
从15名处于增殖期的女性和8名处于分泌期的女性获取子宫肌层、子宫平滑肌瘤外周和中央部分的活检标本。通过Ki67免疫组化染色检测有丝分裂细胞。采用TUNEL法检测凋亡细胞。计算有丝分裂和凋亡指数。使用商用单克隆受体酶免疫分析试剂盒测量雌激素和孕激素受体的组织水平。
在分泌期，平滑肌瘤外周部分的有丝分裂指数显著高于中央部分。在增殖期，外周部分的凋亡指数显著高于中央部分。这些差异未在受体表达差异中体现。
本研究结果表明，人类子宫平滑肌瘤的生长主要发生在月经周期分泌期肿瘤的外周部分。这些发现强调了在进行研究工作取样时保持一致的重要性。
