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2003年北京用于检测SARS冠状病毒的实时聚合酶链反应

Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003.

作者信息

Zhai Junhui, Briese Thomas, Dai Erhei, Wang Xiaoyi, pang Xin, Du Zongmin, Liu Haihong, Wang Jin, Wang Hongxia, Guo Zhaobiao, Chen Zeliang, Jiang Lingxiao, Zhou Dongsheng, Han Yanping, Jabado Omar, Palacios Gustavo, Lipkin W Ian, Tang Ruifu

机构信息

Institute of Microbiology and Epidemiology, Academy of Military Sciences, Beijing, China.

出版信息

Emerg Infect Dis. 2004 Feb;10(2):300-3. doi: 10.3201/eid1002.030799.

DOI:10.3201/eid1002.030799
PMID:15030701
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3322935/
Abstract

During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.

摘要

在2003年严重急性呼吸综合征（SARS）疫情爆发期间，建立了一种针对病毒基因组3'端核衣壳基因的实时定量聚合酶链反应，用于检测和鉴定与SARS相关的冠状病毒。我们描述了使用该检测方法对700多个临床样本进行筛查的情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60d/3322935/357d25897afd/03-0799-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60d/3322935/2529950dde2a/03-0799-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60d/3322935/357d25897afd/03-0799-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60d/3322935/2529950dde2a/03-0799-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60d/3322935/357d25897afd/03-0799-F2.jpg

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Early diagnosis of SARS coronavirus infection by real time RT-PCR.
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