Jiang Wen G, Douglas-Jones Anthony, Mansel Robert E
Metastasis Research Group, University Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK.
Prostaglandins Leukot Essent Fatty Acids. 2003 Oct;69(4):275-81. doi: 10.1016/s0952-3278(03)00110-8.
Lipoxygenases and cyclooxygenase are key mediators of arachidonic acid metabolism. The eicosanoids metabolites from these oxygynases have been shown to regulate the growth and death of cancer cells. This study determined the level of expression of 5-, 12-, 15-lipoxygenase and cyclooxygenase-2 expression in a cohort of breast cancer patients and their correlation with clinical outcomes. Compared with normal breast tissues, tumour tissues exhibited a significantly higher levels of 12-lipoxygenase and cyclooxygenase-2 (P<0.05), and significantly lower level of 15-lipoxygenase (P=0.05). Lobular carcinomas had a higher level of cyclooxygenase-2 and lower level of 15-lipoxygenase than ductal carcinomas. The lowest level of 15-lipoxygenase was seen in TNM3 and TNM4 tumours and from patients who died of breast cancer. Levels of 12- and 5-lipoxygenases were also particularly high in tumours from patients who died of breast cancer. This study shows that human breast tumours aberrantly express lipoxygenases and cyclooxygenase-2 and that decreased level of 15-lipoxygenase and raised level of cyclooxygenase-2 and 12-lipoxygenase has prognostic value in patients with breast cancer.
脂氧合酶和环氧化酶是花生四烯酸代谢的关键介质。这些氧化酶产生的类二十烷酸代谢产物已被证明可调节癌细胞的生长和死亡。本研究测定了一组乳腺癌患者中5-、12-、15-脂氧合酶和环氧化酶-2的表达水平及其与临床结局的相关性。与正常乳腺组织相比，肿瘤组织中12-脂氧合酶和环氧化酶-2的水平显著更高（P<0.05），而15-脂氧合酶的水平显著更低（P=0.05）。小叶癌中环氧化酶-2的水平高于导管癌，而15-脂氧合酶的水平低于导管癌。在TNM3和TNM4期肿瘤以及死于乳腺癌的患者中，15-脂氧合酶的水平最低。在死于乳腺癌的患者的肿瘤中，12-脂氧合酶和5-脂氧合酶的水平也特别高。本研究表明，人类乳腺肿瘤异常表达脂氧合酶和环氧化酶-2，并且15-脂氧合酶水平降低以及环氧化酶-2和12-脂氧合酶水平升高对乳腺癌患者具有预后价值。
