Department of Bromatology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Nutrients. 2021 Oct 27;13(11):3821. doi: 10.3390/nu13113821.
The aim of this study was to investigate the effect of zinc supplementation (in the form of nano or microparticles) on the profile and metabolism of fatty acids in the liver microsomes of rats with induced breast cancer. The activity of desaturases (Δ5, Δ6, Δ9) and the level of cholesterol and its oxidized derivatives were measured. The aim of this study was also to determine the effect of various forms of zinc supplements on rats that were on 5-, 12- and 15-hydroxyeicosatetraenoic (5-, 12- and 15-HETE) and hydroxyoctadecadienoic (HODE) acids, and the level of prostaglandin E2 (PGE2). Female Spraque-Dawley rats ( = 24) were divided into 2 groups that were supplemented with zinc in the micro form (342 nm) or nano form (99 nm) particles, respectively, and a group with a standard diet (control group). All animals received 7,12-dimethylbenz[a]anthracene twice for the induction of breast cancer. Dietary nano-Zn supplementation increased vaccenic acid content ( = 0.032) and decreased Δ6-desaturase activity ( = 0.006), whereas micro-Zn increased cholesterol ( = 0.006), ∑COPs (total cholesterol-oxidation products) ( = 0.019) and PGE2 ( = 0.028) content. Dietary enrichment with Zn microparticles resulted in lower concentrations of the metabolites 15-, 12- and 5-HETE and HODE. Our study indicates that the effect of zinc supplementation on the metabolism of fatty acids in the liver microsomes under neoplastic conditions depends on the form in which it is administered.
本研究旨在探讨锌补充剂（纳米或微粒形式）对诱导乳腺癌大鼠肝微粒体中脂肪酸谱和代谢的影响。测量了去饱和酶（Δ5、Δ6、Δ9）的活性以及胆固醇及其氧化衍生物的水平。本研究还旨在确定不同形式的锌补充剂对 5-、12-和 15-羟基二十碳四烯酸（5-、12-和 15-HETE）和羟基十八碳二烯酸（HODE）酸以及前列腺素 E2（PGE2）水平的影响。雌性 Spraque-Dawley 大鼠（n = 24）分为两组，分别补充微形式（342nm）或纳米形式（99nm）的锌颗粒，以及标准饮食组（对照组）。所有动物均接受两次 7,12-二甲基苯并[a]蒽以诱导乳腺癌。膳食纳米锌补充剂增加了 Vaccenic 酸的含量（p = 0.032），降低了 Δ6-去饱和酶的活性（p = 0.006），而微锌增加了胆固醇（p = 0.006）、∑COPs（总胆固醇氧化产物）（p = 0.019）和 PGE2（p = 0.028）的含量。膳食中锌微颗粒的富集导致代谢物 15-、12-和 5-HETE 和 HODE 的浓度降低。我们的研究表明，在肿瘤条件下，锌补充对肝微粒体中脂肪酸代谢的影响取决于其给药形式。
