Bennett Désirée E, Cafferkey Mary T
Epidemiology and Molecular Biology Unit, The Children's University Hospital, Temple Street, Dublin 1, Ireland 2Department of Clinical Microbiology, Royal College of Surgeons in Ireland, York Street, Dublin 2, Ireland.
J Med Microbiol. 2003 Sep;52(Pt 9):781-787. doi: 10.1099/jmm.0.05225-0.
Invasive disease-associated strains of Neisseria meningitidis were analysed by multilocus restriction typing (MLRT), which involves the restriction fragment-length polymorphism analysis of PCR products generated from the seven loci of housekeeping genes used in MLST. Several different restriction patterns (alleles) were observed for each of the seven loci examined. Greater allelic variation was observed with the fumC and pgm loci than with the abcZ and adk loci, suggesting that the latter were more conserved. The alleles at each of the seven loci were combined to give an allelic profile or restriction type (RT). A good correlation between RT and serogroup, serotype and serosubtype was observed, as all C 2ap1.2,5 strains were contained in a single RT, as were all but one strain of B 4p1.4. In this study, MLRT proved to be an efficient, effective and relatively inexpensive method for N. meningitidis strain characterization.
采用多位点限制性分型（MLRT）对侵袭性疾病相关的脑膜炎奈瑟菌菌株进行分析，该方法涉及对从多位点序列分型（MLST）中使用的管家基因的7个位点产生的PCR产物进行限制性片段长度多态性分析。在所检测的7个位点中，每个位点都观察到了几种不同的限制性模式（等位基因）。与abcZ和adk位点相比，fumC和pgm位点观察到了更大的等位基因变异，表明后者更保守。将7个位点中的每个位点的等位基因组合起来，得到一个等位基因谱或限制性类型（RT）。观察到RT与血清群、血清型和血清亚型之间有良好的相关性，因为所有C 2ap1.2,5菌株都包含在单一的RT中，除一株外所有B 4p1.4菌株也是如此。在本研究中，MLRT被证明是一种用于脑膜炎奈瑟菌菌株鉴定的高效、有效且相对廉价的方法。
