Votava M
Mikrobiologický ústav FN u sv. Anny, Brno.
Cesk Epidemiol Mikrobiol Imunol. 1992 Dec;41(6):317-26.
The new modification (HEA test) is based on the presence of a singularly distinct agglutination of native horse erythrocytes in a serum dilution 1:10 and more from cases with Epstein-Barr virus infectious mononucleosis. In 1665 sera the HEA test was equally often positive as the OCH test (in 5.2%) whereas the IM test with formal-treated horse erythrocytes was positive only in 3.7%. The sensitivity of the IM test in comparison with the OCH test was 61%, the specificity 99.5%. The sensitivity of the HEA test in comparison with the OCH test was 76%, the specificity 98.7%. The specificity of the HEA test in a group of 52 sera without IgG antibodies to EB-viral capsid antigen (EB VCA) was 94%, in another group of 130 sera from hepatitis cases it was 98%. In 103 pairs of sera from infants aged 1-5 years the positivity of the OCH test, HEA test, IgM antibodies to EB VCA and the four-fold rise in the titre of EB-viral IgG were, 20%, 16%, 23% and 24% respectively. Analogous values in 159 pairs of sera from older subjects were 42%, 40%, 38% and 29%. The HEA test is recommended as useful adjunct to the OCH test for the detection of heterophile antibodies even in young children.
新的改良方法（HEA试验）基于在来自爱泼斯坦-巴尔病毒感染性单核细胞增多症患者血清1:10及更高稀释度中天然马红细胞出现明显独特的凝集现象。在1665份血清中，HEA试验阳性率与OCH试验相同（均为5.2%），而用甲醛处理过的马红细胞进行的IM试验阳性率仅为3.7%。与OCH试验相比，IM试验的敏感性为61%，特异性为99.5%。与OCH试验相比，HEA试验的敏感性为76%，特异性为98.7%。在一组52份无EB病毒衣壳抗原（EB VCA）IgG抗体的血清中，HEA试验的特异性为94%，在另一组130份肝炎患者血清中为98%。在103对1至5岁婴儿的血清中，OCH试验、HEA试验、针对EB VCA的IgM抗体阳性率以及EB病毒IgG滴度呈四倍升高的比例分别为20%、16%、23%和24%。在159对年长受试者的血清中，类似的值分别为42%、40%、38%和29%。即使对于幼儿，也推荐将HEA试验作为OCH试验检测嗜异性抗体的有用辅助方法。
