Földes Gábor, Horkay Ferenc, Szokodi István, Vuolteenaho Olli, Ilves Mika, Lindstedt Ken A, Mäyränpää Mikko, Sármán Balázs, Seres Leila, Skoumal Réka, Lakó-Futó Zoltán, deChâtel Rudolf, Ruskoaho Heikki, Tóth Miklós
Ist Department of Medicine, Semmelweis University, Budapest, Hungary.
Biochem Biophys Res Commun. 2003 Aug 29;308(3):480-5. doi: 10.1016/s0006-291x(03)01424-4.
The orphan receptor APJ and its recently identified endogenous ligand, apelin, are expressed in the heart. However, their importance in the human cardiovascular system is not known. This study shows that apelin-like immunoreactivity is abundantly present in healthy human heart and plasma. Gel filtration HPLC analysis revealed that atrial and plasma levels of high molecular weight apelin, possibly proapelin, were markedly higher than those of mature apelin-36 itself. As assessed by quantitative RT-PCR analysis, left ventricular apelin mRNA levels were increased 4.7-fold in chronic heart failure (CHF) due to coronary heart disease (p<0.01) and 3.3-fold due to idiopathic dilated cardiomyopathy (p<0.05), whereas atrial apelin mRNA levels were unchanged. Atrial and plasma apelin-like immunoreactivity as well as atrial and ventricular APJ receptor mRNA levels were significantly decreased in CHF. Our results suggest that a new cardiac regulatory peptide, apelin, and APJ receptor may contribute to the pathophysiology of human CHF.
孤儿受体APJ及其最近发现的内源性配体apelin在心脏中表达。然而，它们在人类心血管系统中的重要性尚不清楚。本研究表明，apelin样免疫反应性在健康人的心脏和血浆中大量存在。凝胶过滤HPLC分析显示，高分子量apelin（可能是proapelin）的心房和血浆水平明显高于成熟的apelin-36本身。通过定量RT-PCR分析评估，冠心病所致慢性心力衰竭（CHF）患者左心室apelin mRNA水平升高4.7倍（p<0.01），特发性扩张型心肌病所致患者升高3.3倍（p<0.05），而心房apelin mRNA水平未改变。CHF患者的心房和血浆apelin样免疫反应性以及心房和心室APJ受体mRNA水平显著降低。我们的结果表明，一种新的心脏调节肽apelin和APJ受体可能参与了人类CHF的病理生理过程。
