Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram-695581, India.
Int Immunopharmacol. 2012 Jan;12(1):205-11. doi: 10.1016/j.intimp.2011.11.012. Epub 2011 Dec 7.
The aim of this study was to investigate anti-inflammatory and antioxidant effects of mucilage from fenugreek in adjuvant induced arthritis in rats. Arthritis was induced by intradermal injection of complete Freund's adjuvant into the right hind paw produce inflammation of the joint. The activities of inflammatory enzymes like cyclooxygenase, lipoxygenase and myeloperoxidase, and levels of nitrite and C-reactive protein were observed. Also oxidative stress was measured by analyzing the activity of catalase, superoxide dismutase, glutathione peroxidase and the levels of glutathione and vitamin C and lipid peroxidation. The blood parameters like ESR, total WBC, RBC and hemoglobin content was checked. Fenugreek mucilage exhibited maximum percentage of edema inhibition at a dose of 75 mg/kg on 21st day of adjuvant arthritis. The effect was higher than that of standard drug indomethacin. The activities of cyclooxygenase-2 and myeloperoxidase and concentration of thiobarbituric acid reactive substance (TBARS) were decreased and the activities of antioxidant enzymes, vitamins C and reduced glutathione level were increased on treatment with fenugreek mucilage. The increment in ESR and total WBC, reduction in RBC count and hemoglobin and aberrant changes to the C-reactive protein (CRP) levels observed in the arthritic animals were also found to be significantly restored in fenugreek mucilage treated rats. Histopathology of paw tissue showed decreased edema formation and cellular infiltration on supplementation with fenugreek mucilage. Thus the results demonstrated the potential beneficiary effect of fenugreek mucilage on adjuvant induced arthritis in rats.
本研究旨在探讨葫芦巴胶对弗氏完全佐剂诱导的大鼠关节炎的抗炎和抗氧化作用。通过向右侧后爪皮内注射完全弗氏佐剂诱导关节炎，产生关节炎症。观察炎症酶（如环加氧酶、脂加氧酶和髓过氧化物酶）的活性以及亚硝酸盐和 C 反应蛋白的水平。还通过分析过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶的活性以及谷胱甘肽和维生素 C 的水平和脂质过氧化来衡量氧化应激。检查血液参数，如 ESR、总白细胞计数、红细胞计数和血红蛋白含量。在弗氏完全佐剂关节炎第 21 天，葫芦巴胶在 75mg/kg 剂量下表现出最大的水肿抑制百分比。其效果高于标准药物吲哚美辛。环加氧酶-2 和髓过氧化物酶的活性以及硫代巴比妥酸反应物质（TBARS）的浓度降低，抗氧化酶、维生素 C 和还原型谷胱甘肽水平的活性增加。在接受葫芦巴胶治疗的大鼠中，还观察到关节炎动物中 ESR 和总白细胞计数的增加、红细胞计数和血红蛋白的减少以及 C 反应蛋白（CRP）水平的异常变化得到了显著恢复。爪组织的组织病理学显示，补充葫芦巴胶可减少水肿形成和细胞浸润。因此，结果表明葫芦巴胶对弗氏完全佐剂诱导的大鼠关节炎具有潜在的有益作用。
