Yazaki Yasuyuki, Mizuo Hitoshi, Takahashi Masaharu, Nishizawa Tsutomu, Sasaki Nobuhiko, Gotanda Yuhko, Okamoto Hiroaki
Center for Gastroenterology, Kobayashi Hospital, Hokkaido 090-8567, Japan.
Department of Internal Medicine, Kin-ikyo Chuo Hospital, Hokkaido 007-0870, Japan.
J Gen Virol. 2003 Sep;84(Pt 9):2351-2357. doi: 10.1099/vir.0.19242-0.
Among ten patients who contracted sporadic acute or fulminant hepatitis E between 2001 and 2002 in Hokkaido, Japan, nine (90 %) had a history of consuming grilled or undercooked pig liver 2-8 weeks before the disease onset. We tested packages of raw pig liver sold in grocery stores as food in Hokkaido for the presence of hepatitis E virus (HEV) RNA by RT-PCR. Pig liver specimens from seven (1.9 %) of 363 packages had detectable HEV RNA. Partial sequence analyses revealed that the seven swine HEV isolates belonged to genotype III or IV. One swine HEV isolate (swJL145) from a packaged pig liver had 100 % identity with the HE-JA18 isolate recovered from an 86-year-old patient in Hokkaido. Two swine HEV isolates (swJL234 and swJL325) had 98.5-100 % identity with the HE-JA4 isolate obtained from a 44-year-old patient in Hokkaido. These results indicate that inadequately cooked pig liver may transmit HEV to humans.
在2001年至2002年间于日本北海道感染散发性急性或暴发性戊型肝炎的10名患者中，9名（90%）在发病前2至8周有食用烤猪或未煮熟猪肝的病史。我们通过逆转录聚合酶链反应（RT-PCR）检测了北海道杂货店作为食品出售的生猪肝包装中戊型肝炎病毒（HEV）RNA的存在情况。363份包装中的7份（1.9%）猪肝标本检测到了可检测到的HEV RNA。部分序列分析表明，这7株猪源HEV分离株属于基因型III或IV。一份包装猪肝中的一株猪源HEV分离株（swJL145）与从北海道一名86岁患者身上分离出的HE-JA18分离株具有100%的同源性。两株猪源HEV分离株（swJL234和swJL325）与从北海道一名44岁患者身上获得的HE-JA4分离株具有98.5%至100%的同源性。这些结果表明，未煮熟的猪肝可能将HEV传播给人类。
