Amisu K O, Coker A O, On S L W, Isokpehi R D
Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria.
East Afr Med J. 2003 Apr;80(4):218-22.
To investigate the prevalence, species distribution and genetic diversity of zoonotic Arcobacter species.
Prospective study.
Drainage system of a cosmopolitan chicken abattoir in Lagos, Nigeria.
One hundred and fifty drainage water samples were enriched in a minimal antibiotics-containing medium at room temperature and bacteria then isolated by use of a membrane filtration method.
Twenty six (14%) of samples were positive for Arcobacter spp. Of these, 20 were examined by a comprehensive probabilistic identification scheme for Epsilobacteria and all strains identified as A. butzleri. AFLP analysis of these strains revealed considerable genetic diversity among the strains, with 12 genotypes defined at the 90% similarity level.
The prevalence of A. butzleri in Nigerian poultry abattoir effluent indicates this species may constitute a public health problem in this country. AFLP profiling could be a useful tool for molecular epidemiological and population genetic studies of this organism. This is the first known report of A. butzleri in Nigeria, and first application of AFLP analysis for genotyping the species.
调查人畜共患弓形杆菌属的流行情况、物种分布及遗传多样性。
前瞻性研究。
尼日利亚拉各斯一个国际化鸡肉屠宰场的排水系统。
150份排水水样在室温下于含最低限度抗生素的培养基中富集，然后使用膜过滤法分离细菌。
26份（14%）样本弓形杆菌属呈阳性。其中，20份通过埃普西隆杆菌综合概率鉴定方案进行检测，所有菌株均鉴定为布氏弓形杆菌。对这些菌株的AFLP分析显示，菌株间存在相当大的遗传多样性，在90%相似性水平下定义了12种基因型。
布氏弓形杆菌在尼日利亚家禽屠宰场废水中的流行表明，该物种可能在该国构成公共卫生问题。AFLP分析可能是该生物体分子流行病学和群体遗传学研究的有用工具。这是尼日利亚首次关于布氏弓形杆菌的已知报告，也是首次将AFLP分析应用于该物种的基因分型。
