Risco Cristina, Carrascosa José L, Frey Teryl K
Department of Structure of Macromolecules, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
Virology. 2003 Aug 1;312(2):261-9. doi: 10.1016/s0042-6822(03)00384-2.
Rubella virus is a small enveloped virus that assembles in association with Golgi membranes. Freeze-substitution electron microscopy of rubella virus-infected cells revealed a previously unrecognized virion polymorphism inside the Golgi stacks: homogeneously dense particles without a defined core coexisting with less dense, mature virions that contained assembled cores. The homogeneous particles appear to be a precursor form during the virion morphogenesis process as the forms with mature morphology were the only ones detected inside secretory vesicles and on the exterior of cells. In mature virions potential remnants of C protein membrane insertion were visualized as dense strips connecting the envelope with the internal core. In infected cells Golgi stacks were frequently seen close to cytopathic vacuoles, structures identified as the sites for viral RNA replication, along with the rough endoplasmic reticulum and mitochondria. These associations could facilitate the transfer of viral genomes from the cytopathic vacuoles to the areas of rubella assembly in Golgi membranes.
风疹病毒是一种小型包膜病毒，其组装与高尔基体膜相关。对风疹病毒感染细胞进行冷冻置换电子显微镜观察发现，在高尔基体堆栈内部存在一种先前未被识别的病毒粒子多态性：均匀致密的颗粒，无明确核心，与密度较低的成熟病毒粒子共存，成熟病毒粒子含有组装好的核心。均匀颗粒似乎是病毒粒子形态发生过程中的一种前体形式，因为具有成熟形态的形式是在分泌小泡内部和细胞外部检测到的唯一形式。在成熟病毒粒子中，C蛋白膜插入的潜在残余物表现为连接包膜与内部核心的致密条带。在感染细胞中，经常可以看到高尔基体堆栈靠近细胞病变空泡，细胞病变空泡被确定为病毒RNA复制的位点，同时还有粗面内质网和线粒体。这些关联可能有助于病毒基因组从细胞病变空泡转移到高尔基体膜中风疹组装区域。
