Tar'an B, Warkentin T, Somers D J, Miranda D, Vandenberg A, Blade S, Woods S, Bing D, Xue A, DeKoeyer D, Penner G
Crop Development Centre, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada.
Theor Appl Genet. 2003 Nov;107(8):1482-91. doi: 10.1007/s00122-003-1379-9. Epub 2003 Aug 15.
With the development of genetic maps and the identification of the most-likely positions of quantitative trait loci (QTLs) on these maps, molecular markers for lodging resistance can be identified. Consequently, marker-assisted selection (MAS) has the potential to improve the efficiency of selection for lodging resistance in a breeding program. This study was conducted to identify genetic loci associated with lodging resistance, plant height and reaction to mycosphaerella blight in pea. A population consisting of 88 recombinant inbred lines (RILs) was developed from a cross between Carneval and MP1401. The RILs were evaluated in 11 environments across the provinces of Manitoba, Saskatchewan and Alberta, Canada in 1998, 1999 and 2000. One hundred and ninety two amplified fragment length polymorphism (AFLP) markers, 13 random amplified polymorphic DNA (RAPD) markers and one sequence tagged site (STS) marker were assigned to ten linkage groups (LGs) that covered 1,274 centi Morgans (cM) of the pea genome. Six of these LGs were aligned with the previous pea map. Two QTLs were identified for lodging resistance that collectively explained 58% of the total phenotypic variation in the mean environment. Three QTLs were identified each for plant height and resistance to mycosphaerella blight, which accounted for 65% and 36% of the total phenotypic variation, respectively, in the mean environment. These QTLs were relatively consistent across environments. The AFLP marker that was associated with the major locus for lodging resistance was converted into the sequence-characterized amplified-region (SCAR) marker. The presence or absence of the SCAR marker corresponded well with the lodging reaction of 50 commercial pea varieties.
随着遗传图谱的发展以及数量性状基因座（QTL）在这些图谱上最可能位置的确定，可以鉴定出抗倒伏的分子标记。因此，标记辅助选择（MAS）有潜力提高育种计划中抗倒伏选择的效率。本研究旨在鉴定与豌豆抗倒伏性、株高和对豌豆壳二孢叶枯病反应相关的基因座。由Carneval和MP1401杂交培育出一个包含88个重组自交系（RIL）的群体。1998年、1999年和2000年，在加拿大曼尼托巴省、萨斯喀彻温省和艾伯塔省的11个环境中对这些RIL进行了评估。192个扩增片段长度多态性（AFLP）标记、13个随机扩增多态性DNA（RAPD）标记和1个序列标签位点（STS）标记被定位到覆盖豌豆基因组1274厘摩（cM）的10个连锁群（LG）上。其中6个LG与先前的豌豆图谱对齐。鉴定出两个抗倒伏QTL，它们共同解释了平均环境中总表型变异的58%。分别鉴定出3个控制株高和抗豌豆壳二孢叶枯病的QTL，在平均环境中它们分别占总表型变异的65%和36%。这些QTL在不同环境中相对一致。与抗倒伏主基因座相关的AFLP标记被转化为序列特征性扩增区域（SCAR）标记。SCAR标记的有无与50个商业豌豆品种的抗倒伏反应高度吻合。
