Alvarez I, Pagán R, Condón S, Raso J
Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, 50.013 Saragossa, Spain.
Int J Food Microbiol. 2003 Oct 15;87(1-2):87-95. doi: 10.1016/s0168-1605(03)00056-4.
The influence of the electric field strength, the treatment time, the total specific energy and the conductivity of the treatment medium on the Listeria monocytogenes inactivation by pulsed electric fields (PEF) has been investigated. L. monocytogenes inactivation increased with the field strength, treatment time and specific energy. A maximum inactivation of 4.77 log(10) cycles was observed after a treatment of 28 kV/cm, 2000 micros and 3490 kJ/kg. The lethal effect of PEF treatments on L. monocytogenes was not influenced by the conductivity of the treatment medium in a range of 2, 3 and 4 mS/cm when the total specific energy was used as a PEF control parameter. A mathematical model based on the Weibull distribution was fitted to the experimental data when the field strength (15-28 kV/cm), treatment time (0-2000 micros) and specific energy (0-3490 kJ/kg) were used as PEF control parameters. A linear relationship was obtained between the log(10) of the scale factor (b) and the electric field strength when the treatment time and the total specific energy were used to control the process. The total specific energy, in addition to the electric field strength and the treatment time, should be reported in order to evaluate the microbial inactivation by PEF.
研究了电场强度、处理时间、总比能和处理介质的电导率对脉冲电场（PEF）灭活单核细胞增生李斯特菌的影响。单核细胞增生李斯特菌的灭活率随电场强度、处理时间和比能的增加而提高。在28 kV/cm、2000微秒和3490 kJ/kg的处理条件下，观察到最大灭活率为4.77 log(10) 个循环。当以总比能作为PEF控制参数时，在2、3和4 mS/cm的电导率范围内，PEF处理对单核细胞增生李斯特菌的致死效应不受处理介质电导率的影响。当以电场强度（15 - 28 kV/cm）、处理时间（0 - 2000微秒）和比能（0 - 3490 kJ/kg）作为PEF控制参数时，基于威布尔分布的数学模型拟合了实验数据。当用处理时间和总比能控制过程时，尺度因子（b）的log(10) 与电场强度之间获得了线性关系。为了评估PEF对微生物的灭活效果，除了电场强度和处理时间外，还应报告总比能。
