Bayley Mark, Larsen Peter Foged, Baekgaard Henrik, Baatrup Erik
Department of Zoology, Institute of Biological Sciences, University of Aarhus, DK-8000 Aarhus C, Denmark.
Biol Reprod. 2003 Dec;69(6):1951-6. doi: 10.1095/biolreprod.103.017780. Epub 2003 Aug 20.
Despite the enormous volume of research concerning the various effects of chemicals with endocrine-disrupting properties in fish, there is still very little evidence that endocrine disruption can adversely affect individual fertility and, hence, pose problems for the population. In the present study, guppies (Poecilia reticulata) were fed with the anti-androgenic fungicide vinclozolin at concentrations ranging from 1.8 to 180 mg/kg from 8-14 wk of age. Male sperm count and the intensity of his sexual display behavior were significantly reduced in treatment groups, which was in line with the results of previous studies. Here, we show further that these impairments translate into reduced fertility, measured as the size of the female's first clutch. Also, this reduced fertility was correlated to the male sperm count, but not to the intensity of the male sexual display. Finally, by crossing exposed with unexposed animals, we show that the adverse effect of vinclozolin on reproduction is mediated through the male alone.
尽管关于具有内分泌干扰特性的化学物质对鱼类的各种影响已有大量研究，但仍几乎没有证据表明内分泌干扰会对个体生育能力产生不利影响，进而给种群带来问题。在本研究中，将孔雀鱼（孔雀鱼）从8至14周龄开始，以1.8至180毫克/千克的浓度喂食抗雄激素杀菌剂乙烯菌核利。处理组雄性的精子数量和其求偶行为的强度显著降低，这与先前的研究结果一致。在此，我们进一步表明，这些损伤转化为生育能力下降，以雌性首次产卵的大小来衡量。此外，这种生育能力下降与雄性精子数量相关，但与雄性求偶行为的强度无关。最后，通过将暴露组与未暴露组动物杂交，我们表明乙烯菌核利对繁殖的不利影响仅通过雄性介导。
