幽门螺杆菌的双组分系统在小鼠感染模型中有助于其致病性。
Two-component systems of Helicobacter pylori contribute to virulence in a mouse infection model.
作者信息
Panthel Klaus, Dietz Patricia, Haas Rainer, Beier Dagmar
机构信息
Max-von-Pettenkofer Institut für Hygiene und Medizinische Mikrobiologie, D-80336 Munich, Germany.
出版信息
Infect Immun. 2003 Sep;71(9):5381-5. doi: 10.1128/IAI.71.9.5381-5385.2003.
Abstract
Helicobacter pylori encodes three histidine kinases and five response regulators belonging to the family of two-component regulatory systems which are involved in transcriptional control. Here we demonstrate that isogenic mutants of H. pylori P76 with deletions of the response regulator open reading frame (ORF) HP1365 and ORFs HP244, HP165, and HP1364 encoding histidine kinases are unable to colonize the stomachs of BALB/c mice, suggesting an essential role of these systems in the regulation of important virulence properties of H. pylori. Furthermore, we demonstrate that the genes under the control of the P(HP1408) and P(HP119) promoters which are regulated by the two-component system HP166-HP165 are not essential for single mutant colonization of mice but are required under competitive colonization conditions.
摘要
幽门螺杆菌编码三种组氨酸激酶和五种响应调节因子，它们属于双组分调节系统家族，参与转录调控。在此，我们证明，幽门螺杆菌P76的同基因突变体，其响应调节因子开放阅读框（ORF）HP1365以及编码组氨酸激酶的ORF HP244、HP165和HP1364缺失，无法在BALB/c小鼠的胃中定殖，这表明这些系统在调节幽门螺杆菌重要毒力特性方面起着至关重要的作用。此外，我们证明，由双组分系统HP166-HP165调控的P(HP1408)和P(HP119)启动子控制下的基因，对于小鼠的单突变体定殖并非必需，但在竞争性定殖条件下是必需的。
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