从铜绿假单胞菌突变体中对多药外排泵mexHI-opmD进行功能克隆及特性分析。
Functional cloning and characterization of a multidrug efflux pump, mexHI-opmD, from a Pseudomonas aeruginosa mutant.
作者信息
Sekiya Hiroshi, Mima Takehiko, Morita Yuji, Kuroda Teruo, Mizushima Tohru, Tsuchiya Tomofusa
机构信息
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700-8530, Japan.
出版信息
Antimicrob Agents Chemother. 2003 Sep;47(9):2990-2. doi: 10.1128/AAC.47.9.2990-2992.2003.
Abstract
We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.
摘要
我们从缺乏四种主要多药外排泵的铜绿假单胞菌YM64中分离出了突变体YM644，该突变体对诺氟沙星、溴化乙锭、吖啶黄素和罗丹明6G表现出更高的抗性。负责这种抗性的基因是mexHI-opmD。携带这些基因的质粒的YM644和YM64细胞观察到溴化乙锭外排增加。YM644染色体DNA中的基因破坏使细胞对这些药物敏感。
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