基于纸片扩散法测定近平滑念珠菌对阿尼芬净的敏感性。
Disk diffusion-based methods for determining Candida parapsilosis susceptibility to anidulafungin.
作者信息
Odabasi Zekaver, Paetznick Victor, Goldstein Beth P, Rex John H, Ostrosky-Zeichner Luis
机构信息
Division of Infectious Diseases, University of Texas-Houston Medical School, Houston, Texas 77030, USA.
出版信息
Antimicrob Agents Chemother. 2003 Sep;47(9):3018-20. doi: 10.1128/AAC.47.9.3018-3020.2003.
Abstract
Zone diameters for anidulafungin by disk diffusion for 139 isolates of C. parapsilosis were compared with MICs by NCCLS M27-A2 broth microdilution. The comparison was poor unless the disks were prepared by dissolving anidulafungin in 1% dimethyl sulfoxide plus 0.1% Tween 80 and testing on Mueller-Hinton agar flooded with glucose and methylene blue.
摘要
通过纸片扩散法对139株近平滑念珠菌进行阿尼芬净的抑菌圈直径测定，并与采用NCCLS M27 - A2肉汤微量稀释法测定的最低抑菌浓度（MIC）进行比较。除非将阿尼芬净溶解于1%二甲基亚砜加0.1%吐温80中制备纸片，并在添加了葡萄糖和亚甲蓝的Mueller - Hinton琼脂上进行测试，否则两者的比较结果不佳。
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