Pfaller M A, Barry A, Bille J, Brown S, Ellis D, Meis J F, Rennie R, Rinaldi M, Rogers T, Traczewski M
Medical Microbiology Division, Department of Pathology, University of Iowa, Iowa City, Iowa 52242, USA.
J Clin Microbiol. 2004 Apr;42(4):1716-8. doi: 10.1128/JCM.42.4.1716-1718.2004.
An international collaborative study was performed in order to propose quality control limits for voriconazole disk diffusion tests on Mueller-Hinton agar with 2% glucose and 0.5 micro g of methylene blue per ml. The supplement may be added to the agar before autoclaving, or Mueller-Hinton agar plates may be flooded with a glucose-methylene blue solution. Replicate tests on both types of agar plates with 1- micro g voriconazole disks generated data to propose zone size limits for tests of Candida parapsilosis ATCC 22019 (28 to 37 mm), Candida albicans ATCC 90028 (31 to 42 mm), and Candida krusei ATCC 6258 (16 to 25 mm). Candida tropicalis ATCC 750 was not useful for this purpose.
开展了一项国际合作研究，目的是为在含2%葡萄糖和每毫升0.5微克亚甲蓝的穆勒-欣顿琼脂上进行伏立康唑纸片扩散试验提出质量控制限度。该补充剂可在高压灭菌前添加到琼脂中，或者穆勒-欣顿琼脂平板可用葡萄糖-亚甲蓝溶液覆盖。使用1微克伏立康唑纸片在两种类型的琼脂平板上进行重复试验，生成的数据可用于提出近平滑念珠菌ATCC 22019（28至37毫米）、白色念珠菌ATCC 90028（31至42毫米）和克柔念珠菌ATCC 6258（16至25毫米）试验的抑菌圈大小限度。热带念珠菌ATCC 750不适用于此目的。
