Doytchinova Irini, Flower Darren
Edward Jenner Institute for Vaccine Research, Compton, Berkshire, UK RG20 7NN.
Org Biomol Chem. 2003 Aug 7;1(15):2648-54. doi: 10.1039/b300707c.
Identification of epitopes capable of binding multiple HLA types will significantly rationalise the development of epitope-based vaccines. A quantitative method assessing the contribution of each amino acid at each position was applied to over 500 nonamer peptides binding to 5 MHC alleles--A0201, A0202, A0203, A0206 and A6802--which together define the HLA-A2-like supertype. FXIGXI (L)IFV was identified as a supermotif for the A2-supertype based on the contributions of the common preferred amino acids at each of the nine positions. The results indicate that HLA-A6802 is an intermediate allele standing between A2 and A3 supertypes: at anchor position 2 it is closer to A3 and at anchor position 9 it is nearer to A2. Models are available free on-line at http://www.jenner.ac.uk/MHCPred and can be used for binding affinity prediction.
鉴定能够结合多种HLA类型的表位将显著使基于表位的疫苗研发合理化。一种评估每个位置上每个氨基酸贡献的定量方法应用于500多个与5个MHC等位基因（A0201、A0202、A0203、A0206和A6802）结合的九肽，这些等位基因共同定义了HLA - A2样超型。基于九个位置上每个位置常见的优选氨基酸的贡献，FXIGXI (L)IFV被鉴定为A2超型的超级基序。结果表明，HLA - A6802是介于A2和A3超型之间的中间等位基因：在锚定位置2它更接近A3，在锚定位置9它更接近A2。模型可在http://www.jenner.ac.uk/MHCPred免费在线获取，可用于结合亲和力预测。
